卵巢上皮性肿瘤组织中caspase-3,bcl-2的表达及其与细胞凋亡和增殖的关系

目的探讨caspase-3,bcl-2与卵巢上皮性肿瘤发生、发展的关系.方法利用免疫组化(SP)法和TdT介导的dUTP的缺口末端标记(TUNEL)技术,检测72例卵巢上性肿瘤组织中caspase-3和bcl-2蛋白、PCNA的表达水平及细胞凋亡情况.结果 caspase-3在卵巢恶性肿瘤中的表达(44.2%),明显低于良性肿瘤(83.3%,P＜0.05);在低分化卵巢癌中的表达(76.7%),明显高于高分化卵巢癌(47.1%,P＜0.05).bcl-2蛋白在卵巢恶性肿瘤中的表达(76.6%),明显高于良性肿瘤(25.0%,P＜0.01);在低分化卵巢癌中的表达(42.1%),明显低于高分化卵巢癌(90.0%,P＜0.01).凋亡指数与增殖指数呈正相关(r=0.482,P＜0.01),与caspase-3的表达呈正相关(r=0.647,P＜0.01),与bcl-2的表达呈负相关(r=-0.531,P＜0.01).结论 caspase-3,bcl-2与卵巢上皮性肿瘤的细胞凋亡密切相关,参与细胞凋亡的调节机制,在卵巢上皮性肿瘤恶变的过程中发挥作用,并与恶性程度关系密切,有可能成为卵巢癌预后评估的参考.

Expression of Caspase -3 and Bcl -2 Protein in Epithelial Ovarian Tumor and Relation of The Expression with Cell Apoptosis and Proliferation

Objective: To explore the relation of caspase-3 and bcl-2 protein with the occurrence and development of epithelial ovarian tumor. Methods: Seventy-two cases of primary ovarian tumor were studied by using immunohistochemistry and TdT-mediated dUTP nick end labeling (TUNEL) method, to examine the expression of caspase-3, bcl-2, proliferating cell antigen (PCNA) and the condition of cell apoptosis. Results: The positive rate of caspase-3 in malignant ovarian tumor was 44.2%, which was obviously lower than the expression in benign tumor (83.3%, P<0.05). The expression of caspase-3 in malignant ovarian tumor was correlated with its differentiation and stage (P<0.01). The positive rate of bcl-2 in malignant ovarian tumor was significantly higher than in benign tumor (P<0.01). while decreased with the decreasing of malignant ovarian tumor's differentiation (P<0.01). Apoptosis index correlated positively with proliferating index (r=0.482, P<0.01) and the expression of caspase-3 (r=0.647, P<0.01), while inversely with the expression of bcl-2 (r=-0.531, P<0.01). Conclusion: There is close relation between the expression of caspase-3, bcl-2protein and cell apoptosis in epithelial ovarian tumor. caspase-3, bcl-2 may participate in the regulating mechanism of cell apoptosis and have function in changing malignance. The expression of caspase-3 and bcl-2 protein have close relation with malignant degree of ovarian cancer and might be reference of estimating the prognosis.