择性结扎节段动脉致局灶性脊髓缺血损伤动物模型的建立

背景：以往研究表明采用腹膜后入路结扎兔所有腰动脉的方法能够建立稳定的脊髓缺血损伤动物模型，但动脉的具体结扎部位尚不明确。 目的：采用选择性节段动脉结扎方法建立局灶性脊髓缺血动物模型。 方法：将成年新西兰大白兔16只随机分为对照组和模型组，8只/组。模型组采用腹膜后入路结扎L1~3双侧腰动脉，对照组不结扎血管。建模后第3天，用改良Tarlov评分法评价两组兔神经功能，取结扎血管所对应的脊髓组织并进行苏木精-伊红染色，观察其组织学改变。 结果与结论：模型组后肢出现功能障碍，Tarlov评分低于对照组(P < 0.05)。脊髓组织出现急性炎性改变及神经细胞坏死等脊髓缺血现象。实验结果表明采用腹膜后入路结扎L1~3双侧腰动脉能够建立较稳定的局灶性脊髓缺血损伤模型。

Segmental artery ligation for establishment of local spinal cord ischemic injury model in rabbits

BACKGROUND: Previous studies showed that a stable model of spinal cord injury can be established using a method of lumbar arteries ligation in retroperitoneum. However, the precise ligation area is unclear. OBJECTIVE: To establish a rabbit model of local spinal cord ischemic injury produced by segmental artery ligation. METHODS: A total of 16 adult rabbits were randomly divided into the control and model groups, with 8 animals in each group. Rabbits in the model group experienced ligation of segment artery in retroperitoneum. There was no treatment in the control group. The nerve function of spinal cord was evaluated by modified Tarlov score and the pathological changes of lumbar segmemal spinal cord were observed with the hematoxylin-eosin staining at 3 days after operation. RESULTS AND CONCLUSIONS: Paralysis was evidenced in the model group and the Tarlov scores were smaller than that of the control group (P < 0.05). Acute inflammatory lesion and neuronal necrosis were observed in the model group. Results showed that a stable model of local spinal cord ischemic injury can be established via ligating L1-3 lumbar arteries approach retroperitoneum.