| 基因重叠延伸拼接PCR法钩建骨形态发生蛋白成熟肽真核表达载体的研究 |
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| 引用本文： | 段小红,陈苏民,柴玉波,徐可为.基因重叠延伸拼接PCR法钩建骨形态发生蛋白成熟肽真核表达载体的研究[J].中国组织工程研究与临床康复,2002,6(24):3762-3763. |
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| 作者姓名： | 段小红 陈苏民 柴玉波 徐可为 |
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| 作者单位： | SchoolofMaterialEngineering，Xi'anJiaotongUniversity，TheFourthMilitaryMedicalUniversity，TheFourthMilitaryMedicalUniversity，SchoolofMaterialEngineering，Xi'anJiaotongUniversity Xi'an710049，China，Xi'an710032，China，Xi'an710032，China，Xi'an710049，China |
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| 摘 要： | Structurallyspeaking,BMPsallbelongtotheTGF-βsuper-familyexceptBMP-1.ThefirstBMPwasisolatedfrombovinebonesandwasprovedtohavetheabilitytoinducecartilageformationandboneformationandalsoplayanimportantroleintherepairofboneandcartilage犤1犦.TheresearchontheexpressionofBMP2hastakenitsshape.Inprokaryoticexpressionsystem,mostlytheresearchfo-cusesonexpressionofthematurepeptideofBMP2whileineu-karyoticexpressionsystem,researchfocusesontheexpressionofBMP2full-lengthgeneinCHOcells犤2,3犦.Thereis…
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Construction of a eukaryote expression vector containing bone morphogenetic protein-2 mature peptide by SOE-PCR method |
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| DUAN Xiao-hong ,CHEN Su-min ,CHAI Yu-bo ,XU Ke-wei.Construction of a eukaryote expression vector containing bone morphogenetic protein-2 mature peptide by SOE-PCR method[J].Journal of Clinical Rehabilitative Tissue Engineering Research,2002,6(24):3762-3763. |
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| Authors: | DUAN Xiao-hong CHEN Su-min CHAI Yu-bo XU Ke-wei |
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| Institution: | 1. School of Material Engineering,Xi'an Jiaotong University,Xi'an 710049,China
2. The Fourth Military Medical University,Xi'an 710032,China |
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| Abstract: | Objective To construct an eukaryote expressio n vector containing bone morphogenetic protein-2(BMP 2)mature peptide.Methods Gene splicing by overlapping extension PCR(SOE-PCR)method was used to clone BMP2signal peptide and mature peptide and their fusion fragment.The fusion fragmen t was cloned into an eukaryote expressing vector pcDNA3.1/myc-His(-)A.The sequence of the fusion fragment of BMP2signal peptide and mature peptide was identified.Results The sequence of the fusion fragment was correct comparing with BMP2signal peptide and mature pepti de published by NCBI.Conclusion The vector pcDNA3.1/myc-His(-)A-BMP2sm constructed in this experiment was suitable to applying in eukaryotic expression of BMP2. |
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| Keywords: | BMP2 signal peptide mature pepti de eukaryote expressing vector |
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