食管癌中EGFRvⅢ、MMP-2的表达特点及意义

目的：探讨表皮生长因子受体Ⅲ型突变体（epidermal growth factor receptor variantⅢ，EGFRvⅢ）及基质金属蛋白酶2（matrix metalloproleinase-2，MMP-2）在食管癌组织中的表达与食管癌发生、发展的关系。方法：收集66例人食管癌肿瘤组织，免疫组织化学及蛋白印迹实验检测EGFRvⅢ表达、免疫组织化学检测MMP-2表达，评价EGFRvⅢ、MMP-2在食管癌病人的性别、年龄、TNM分期、病理分级等临床参数组中的表达分布。Pearson相关性检验分析EGFRvⅢ与MMP-2表达相关性。结果：免疫组化显示EGFRvⅢ、MMP-2在肿瘤组织表达的平均灰度净值分别为22．46±4．21、37．46±6．18，在瘤旁正常食管组织平均灰度净值分别为5．54±3．01、9．80±2．24，肿瘤组织与正常组织相比均有显著的统计学差异（P〈0．05）。Western—blot显示EGFRvⅢ在肿瘤和正常组织表达的平均灰度净值分别为0．83±0．15、0．083±0．049，两组相比具有显著的统计学差异（t=9．362，P〈0．001）。EGFRvⅢ与MMP-2相关性系数r=0．766，P〈0．001，成正性线性相关；在不同性别、年龄、肿瘤大小、生长方式组中EGFRvⅢ、MMP-2的表达分布均无统计学差异（P〉0．05），而在不同TNM分期、病理分级和淋巴结转移中均有统计学差异（P〈0．05）。结论：EGFRvⅢ在食管癌中特异性表达，MMP-2与EGFRvⅢ在食管癌中的表达呈显著线性正相关，提示MMP-2与EGFRvⅢ与肿瘤发生发展密切相关。

Expression and significance of epidermal growth factor receptor variant Ⅲ and matrix metalloproteinase- 2, in human esophageal carcinoma

Objective ：To detect the expression and relation of epidermal growth factor receptor variant Ⅲ （EG- FRv Ⅲ ） and matrix metalloproteinase - 2 （ MMP - 2 ） in the genesis and development of human esophageal carcinoma. Methods： In 66 human esophageal carcinoma tissues, the expression of EGFRv Ⅲ was detected by immunohistochemistry and Western - blot. MMP - 2 was detected by immunohistochemistry. The relation between various clinicopathologic factors and EGFRvⅢ, MMP -2 was analyzed. Correlation analysis between EGFRvⅢ and MMP -2 was analyzed by Pearson correlation coefficient. Results： The average gray scale values of EGFRv Ⅲ in esophageal carcinoma and normal tissues by immunohistochemistry were 22.46 ± 4.21 and 5.54 ± 3.01, which showed a significant difference （t =6. 701 ,P 〈0. 001 ） . The average gray scale values by Western - blot were 0.83±0. 15 and 0. 083 ± 0.049 ,which had a significant difference （t = 9. 362 ,P 〈 0.001 ）. The average gray scale values of MMP- 2 in esophageal carcinoma and normal tissues by immunohistochemistry were 37.46 ± 6. 18 and 9.80 ± 2.24, which showed a significant difference （ t = 8. 213 , P 〈 0. 001 ） . Correlation analysis between EGFRvⅢ and MMP -2 showed r = 0. 917 , P 〈 0. 001, suggesting a good positive correlation. The significant differences were observed in TNM - stage, lymph node metastasis and classification in both EGFRvⅢ （ P 〈 0.05 ） and MMP - 2 （ P 〈 0.05 ）, and no obvious difference in sex, age, tumor size, growing type （ P 〉 0.05 ）. Conclusion： EGFRv Ⅲ express in human esop hageal carcinoma. Combination of EGFRvⅢ and MMP - 2 could be useful markers for tumorgenesis and differentiation of human esophageal carcinoma.