2009年广州地区季节性甲型流感病毒对烷胺类药物耐药性分析

目的 分析2009年1-10月份广州地区季节性甲型流感病毒对烷胺类药物的耐药情况,为临床预防和治疗季节性甲型流感提供参考依据.方法 选取2009年1-10月份广州地区6家医院分离的季节性甲型流感病毒株311株,其中H1亚型159株,H3亚型152株.对病毒的毒株基质蛋白(MP)基因进行序列测定,利用数据库和软件进行生物信息分析,并测定软件随机抽取的临床分离的H1和H3病毒株(各10株)对金刚烷胺的药物敏感度,分析广州地区2009年1-10月份季节性甲型流感病毒的耐药性和基因重组情况.结果 159株H1亚型流感病毒中148株对金刚烷胺耐药(93.1%);152株H3型流感病毒均对金刚烷胺耐药(100%);除1株H1亚型流感病毒发生V27A/S31N双重位点突变外,其余均为S31N单位点突变;159株H1亚型流感病毒中MP基因B型(人类)97株,F型(欧洲/澳洲鸟类)62株;152株H3亚型病毒的MP基因均为B型.结论 广州地区季节性甲型流感病毒对烷胺类药物的耐药比例高,且H1亚型流感病毒与欧洲/澳洲鸟类流感病毒可能存在MP基因交换.

Abstract：

Objective To study the prevalence of adamantane-resistance among influenza A viruses isolated from Guangzhou between January and October in 2009, and to provide more information for clinical usage of adamantine drugs. Methods Totally 311 influenza A strains isolated from 6 hospitals in Guangzhou between January and October in 2009 were selected, and the MP gene of all 311 strains ( 159 strains of H1subtype, 152 strains of H3 subtype ) was sequenced. The susceptibility of viruses to rimantadine was assayedby biological methods in cells. Result A hundred and forty-eight strains of influenza A ( H1 ) viruses( 93. 1%, 148/159 ) were resistant to the adamantanes, and all the 152 influenza A ( H3 ) viruses were resistant to the adamantanes. An amino acid substitution S31N was found in most of the strains except 1strain with double mutation V27A/S31N. Furthermore, the M gene of influenza A ( H1 ) viruses was divided into genotype B ( human ) ( 97/159 ) and genotype F ( European and Australian birds, 62/159 ), while the M gene of influenza A ( H3 ) viruses was genotype B ( human ) ( 152/152 ). Conclusion Resistance rate of seasonal influenza A viruses isolated from Guangzhou was high. The MP gene of influenza A ( H1 ) may be replaced by a gene from European and Australian birds through a reassortment event.

Adamantane resistance among seasonal influenza A viruses between January to October in Guangzhou, 2009

Objective To study the prevalence of adamantane-resistance among influenza A viruses isolated from Guangzhou between January and October in 2009, and to provide more information for clinical usage of adamantine drugs. Methods Totally 311 influenza A strains isolated from 6 hospitals in Guangzhou between January and October in 2009 were selected, and the MP gene of all 311 strains ( 159 strains of H1subtype, 152 strains of H3 subtype ) was sequenced. The susceptibility of viruses to rimantadine was assayedby biological methods in cells. Result A hundred and forty-eight strains of influenza A ( H1 ) viruses( 93. 1%, 148/159 ) were resistant to the adamantanes, and all the 152 influenza A ( H3 ) viruses were resistant to the adamantanes. An amino acid substitution S31N was found in most of the strains except 1strain with double mutation V27A/S31N. Furthermore, the M gene of influenza A ( H1 ) viruses was divided into genotype B ( human ) ( 97/159 ) and genotype F ( European and Australian birds, 62/159 ), while the M gene of influenza A ( H3 ) viruses was genotype B ( human ) ( 152/152 ). Conclusion Resistance rate of seasonal influenza A viruses isolated from Guangzhou was high. The MP gene of influenza A ( H1 ) may be replaced by a gene from European and Australian birds through a reassortment event.