转化生长因子β1及Ki-67核抗原在涎腺黏液表皮样癌中的表达和意义

目的 研究转化生长因子β1(transforming growth factor-β1,TGF-β1)、Ki-67核抗原在涎腺黏液表皮样癌(mucoepidermoid carcinoma,MEC)中的表达和意义.方法 应用免疫组织化学SP法检测20例涎腺多形性腺瘤(pleomorphic adenoma,PA)、45例涎腺MEC(29例高分化、16例中低分化)组织中TGF-β1和Ki-67核抗原的表达.结果 涎腺MEC中TGF-β1(39/45)、Ki-67核抗原(43/45)的表达高于PA,差异有统计学意义(P＜0.05);TGF-β1在中低分化MEC中的表达(16/16)高于高分化MEC(23/29)及PA(12/20),差异有统计学意义(P＜0.05),而TGF-β1在高分化MEC(6/23)与PA(8/12)中的表达差异无统计学意义(P＞0.05);在MEC中Ki-67核抗原的表达随TGF-β1表达增强而上升(P＜0.05),二者呈显著正相关(r=0.55,＜0.001).结论 TGF-β1的高表达在涎腺MEC的分化及恶性增殖过程中可能起重要作用.

Abstract：

Objective To investigate the expression and significance of transforming growth factor-β1 (TGF-β1)and Ki-67 nuclear antigen in salivary gland mucoepidermoid carcinoma. Methods The expression of TGF-β1 and Ki-67 nuclear antigen in 20 cases of salivary gland pleomorphic adenoma and 45 cases of mucoepidermoid carcinoma were detected by the streptavidin-peroxidase immunohistochemical method. Results Expression of TGF-β1 (39/45) and Ki-67 nuclear antigen (43/45) in mucoepidermoid carcinoma was significantly higher than in pleomorphic adenoma ( P ＜ 0.05 ). TGF-β1 expression was significantly higher in moderately/poorly differentiated mucoepidermoid carcinoma ( 16/16 ) than that in well differentiated mucoepidermoid carcinoma (23/29) and pleomorphic adenoma (12/20) ( P ＜ 0.05 ), but not different between well differentiated mucoepidermoid carcinoma (6/23) and pleomorphic adenoma (8/12)(P ＞ 0.05 ). The expression of Ki-67 was increased with the increment of TGF-β1 expression in mucoepidermoid carcinoma (P ＜ 0.05 ). Conclusions The high expression of TGF-β1 may play an important role in cell differentiation and malignant proliferation of mucoepidermoid carcinoma.