计算机图像处理系统用于血管平滑肌细胞形态定量分析

采用计算机图像分析法，定量测定家兔培养主动脉血管平滑肌细胞的形态学参数及其与ＤＮＡ合成指标的相关关系，并在此基础上，从细胞形态学角度探讨了中药制剂川芎嗪对ＶＳＭＣ增殖的影响，以评价计算机图像分析技术在细胞学研究中的作用，建立一种研究ＶＳＭＣ增殖水平的新方法。

Application of Computerized Image Analysing System in Morphometric Study of Vascular Smooth Muscle Cells

The authors used image analyser to measure several morphometric parameters of cultured aorta vascular smooth muscle cell (VSMC) and assess the relationships between these morpbometric parameters and 3H-TdR incorporations.On this basis,the authors also explored the effect of Ligustrazine,a traditional Chinese medicine,on VSMC morphology in order to evaluate the role of computerized image analysing system in the study of VSMC proliferation. The results showed that (1) there were significant correlations between VSMC cellular area, nuclear area and 3H-TdR incorporations in control group; (2) Ligustrazine significant;y decreased VSMC cellular area,nuclear area and nuclear perimeter,and a significant correlation was also noted between cellular area and 3H-TdR incorporation in Ligustrazine group. These suggest that computerized image analysing system is a rapid,accurate and reliable method to study the morphology and proliferation of VSMC.