多天线癌胚抗原检测方法的建立及其在恶性肿瘤患者血清中的水平

目的 :建立多天线癌胚抗原 (multipleantennaecarcinoembryonicantigen ,MA CEA)的快速定量检测法 ,并研究肿瘤患者血清中多天线癌胚抗原的水平。方法 :采用神经氨酸酶 (neuramin-idase ,NMD)降解MA CEA糖链外侧的唾液酸。采用蔓陀萝凝集素 (daturastramoniumagglutinin ,DSA)和CEA抗体 ,建立检测MA CEA的链霉亲和素 生物素系统酶免疫分析法 (ABC EIA) ,分别检测 2 39例肺癌、胃癌、肝癌、结肠癌和卵巢癌患者血清中MA CEA的水平 ,同时采用ELISA检测血清CEA的水平。结果 :血清MA CEA的水平在CEA阳性的肺癌患者中明显升高 ,75例肺癌患者血清MA CEA的阳性率为 14 .7% ,而CEA水平升高的肺癌阳性率为 4 7.3% ;但胃癌、肝癌、结肠癌和卵巢癌患者 ,无论CEA水平是否升高 ,均未检测到MA CEA水平的升高。结论 :建立了一种可定量检测肿瘤患者血清MA CEA的方法 ,为肺癌的鉴别诊断及其他多天线糖链肿瘤标志物的研究奠定了基础

Establishment of a method to detect multiple antennae carcinoembryonic antigen (MA-CEA) and MA-CEA level in the sera of patients with malignant tumors

AIM: To establish a rapid quantitative method to detect the serum multiple antennae carcinomabrinic antigen (MA-CEA), and to measure the MA-CEA levels in the sera of patients with malignant tumors. METHODS: Neuraminidase (NMD) was used to digest the sialic acid at terminals of sugar chains of MA-CEA, and then the datura stramonium agglutinin (DSA) and anti-CEA antibody were used to set up streptavidin-biotin complex (ABC) system EIA (ABC-EIA) for detecting serum MA-CEA levels of 239 patients with carcinomas of lung, stomach, liver, colon and ovary. The serum CEA level was determined by ELISA. RESULTS: The level of serum MA-CEA was remarkably elevated in the CEA-positive patients with lung cancer. The positive rate of MA-CEA was 14.6% in 75 patients with lung cancers, and 47.3% in 19 CEA-positive patients with lung cancers. The MA-CEA was not detected in the sera of other tumor patients. CONCLUSION: We have developed a method for detection of MA-CEA levels, which lays the foundation for the differential diagnosis of lung cancer and for research on the other multiple antennae tumor markers.