| Abstract: | Pollen extracts from Atriplex latifolia, Beta vulgaris, Salsola kali and Amaranthus retroflexus were compared with an extract from Chenopodium album by both in vivo and in vitro methods. Skin prick tests on 20 C. album-sensitive patients were positive with all extracts. RAST inhibition together with two-dimensional immunoelectrophoresis and two-dimensional radioimmunoelectrophoresis indicate that common allergenic determinants are present. Electrophoretic transfer for detection of IgE binding molecules from sodium dodecyl sulfate-polyacrylamide gel electrophoresis or isoelectricfocusing gels suggests that the common allergenic determinants are present in molecules with various molecular weights or isoelectric points. |
