| 丙戊酸钠抑制NB4细胞增殖及诱导凋亡的研究 |
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| 引用本文： | 田文亮,朱翠敏,赵蕾,孙立新,张志华,郝长来.丙戊酸钠抑制NB4细胞增殖及诱导凋亡的研究[J].承德医学院学报,2009,26(2). |
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| 作者姓名： | 田文亮 朱翠敏 赵蕾 孙立新 张志华 郝长来 |
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| 作者单位： | 1. 承德医学院,河北承德,067000
2. 承德医学院附属医院 |
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| 摘 要： | 目的:观察组蛋白去乙酰化酶抑制剂丙戊酸钠(VPA)对NB4细胞的作用.方法:台盼蓝细胞计数检测不同浓度VPA对NB4细胞增殖的抑制作用,应用流式细胞术检测VPA作用后NB4细胞CD11b的表达,用DNA梯形片段电泳分析细胞凋亡.结果:VPA能显著抑制NB4细胞的增殖(P＜0.01),VPA处理NB4细胞72h时的半数抑制浓度(IC50)为1.17mmol/L;VPA组NB4细胞髓系分化抗原CD11b表达水平明显高于对照组(P＜0.05);NB4细胞经不同浓度VPA处理48h后,出现典型的凋亡细胞所具有的梯形DNA条带.结论:VPA能抑制NB4细胞增殖,诱导细胞分化和凋亡.
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| 关 键 词： | 丙戊酸钠 组蛋白去乙酰化酶 白血病 细胞周期 细胞凋亡 |
STUDY ON THE EFFECTS OF VALPROIC ACID SODIUM ON INHIBITING PROLIFERATION AND INDUCING APOPTOSIS OF NB4 CELLS |
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| TIAN Wen-liang,ZHU Cui-min,ZHAO Lei,et al.STUDY ON THE EFFECTS OF VALPROIC ACID SODIUM ON INHIBITING PROLIFERATION AND INDUCING APOPTOSIS OF NB4 CELLS[J].Journal of Chengde Medical College,2009,26(2). |
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| Authors: | TIAN Wen-liang ZHU Cui-min ZHAO Lei |
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| Institution: | TIAN Wen-liang,ZHU Cui-min,ZHAO Lei,et al (Chengde Medical College,Hebei Chengde 067000,China) |
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| Abstract: | Objective:To observe the effects of valproic acid sodium(VPA) on NB4 cells.Methods:Trypan blue cell counting was used to detect the effects of VPA in different density on proliferation of NB4 cells;Flow cytometry to observe CD11b expression of NB4 cells;DNA fragment ladder electrophoresis to observe apoptosis of NB4 cells.Results:VPA could obviously inhibit proliferation of NB4 cells(P<0.01);The IC_(50) was 1.17 mmol/L treated with VPA for 72h.In VPA group,the CD11b expression of NB4 cells was significantly... |
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| Keywords: | Valproic acid sodium Histone deacetylases Leukemia Cell cycle Cell apoptosis |
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