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| 一氧化氮与肝硬化心肌病的关系研究 | |
| 引用本文： | 谭凯,张鹏宇,王天才,梁扩寰.一氧化氮与肝硬化心肌病的关系研究[J].华中科技大学学报(医学版),2002,31(2):150-152. |
| 作者姓名： | 谭凯 张鹏宇 王天才 梁扩寰 |
| 作者单位： | 1. 武汉科技大学医学院临床医学系内科教研室,武汉,430081 2. 上海市第一人民医院呼吸内科,上海,200080 3. 华中科技大学同济医学院附属同济医院消化内科,武汉,430030 |
| 基金项目： | 国家自然科学基金资助项目 (No. 396 70 34 8) |
| 摘 要： | 为探讨肝硬化心肌病的发生机制，应用放射性免疫分析法（RIA）测定胆管结扎（BDL）诱导的胆汁性肝硬化大鼠和假手术大鼠血浆及心脏组织匀浆中一氧化氮（NO）依赖性环－磷酸鸟苷（cGMP)深度，采用免疫组织化学法对上述2组大鼠心脏内皮型一氧化氮合酶（eNOS)和诱导型一氧化氮合酶(iNOS)的表达进行比较性研究。结果显示：肝硬化大鼠血浆和心脏组织匀浆中cGMP水平显著高于假手术组（P＜0．01）；肝硬化和假手术大鼠心脏eNOS均呈阳性表达，2组之间无显著性差异，而iNOS在肝硬化大鼠心脏呈强阳性表达，在假手术组则为阴性。提示肝硬化大鼠血浆和心脏组织中NO含量显著增加，而增加的NO主要由iNOS诱导产生，并由此推断一氧化氮途径可能在肝硬化心肌病的发生中起重要作用。 |
| 关 键 词： | 一氧化氮 肝硬化 心肌病 环－磷酸鸟苷 免疫组织化学法 |
| 修稿时间： | 2001年1月9日 |
Relationship between Nitric Oxide and Cirrhotic Cardiomyopathy |
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| Tan Kai ,Zhang Pengyu ,Wang Tiancai et al.Relationship between Nitric Oxide and Cirrhotic Cardiomyopathy[J].Journal of Huazhong University of Science and Technology(Health Sciences),2002,31(2):150-152. | |
| Authors: | Tan Kai Zhang Pengyu Wang Tiancai |
| Institution: | Tan Kai 1,Zhang Pengyu 2,Wang Tiancai 1 et al 1 Department of Internal Medicine,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030 2 Department of Internal Medicine,Shanghai First Peopl |
| Abstract: | To investigate the pathogenesis of cirrhotic cardiomyopathy, concentration of cGMP in plasma and homogenates of cardiac tissue of cirrhotic rats (CIR) induced by bile duct ligation (BDL) and sham-operated rats (SO) was measured by radioimmunoassay (RIA). Controlled studies on the expression of eNOS and iNOS in heart of two groups of rats were undertaken using immunohistochemistry. It was found that the level of cGMP in plasma and homogenates of cardiac tissue of CIR rats was significantly higher than in the SO rats (P<0.01); The expression of eNOS in heart of two groups of rats was all positive, the expression of iNOS in heart of CIR rats was strong positive, but it was negative in SO rats. It was suggested that content of NO in plasma and cardiac tissue of CIR rats was significantly increased, and the increased NO was induced by iNOS. It was concluded that NO-approach may play an important role in the pathogenesis of cirrhotic cardiomyopathy. |
| Keywords: | nitric oxide cirrhotic cardiomyopathy cGMP immunohistochemistry |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |