| 中药遗传毒性研究思路和方法 |
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| 引用本文： | 韩佳寅,易艳,梁爱华,张宇实,李春英,赵雍,王连嵋,李桂琴.中药遗传毒性研究思路和方法[J].中国中药杂志,2015,40(14):2696-2700. |
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| 作者姓名： | 韩佳寅 易艳 梁爱华 张宇实 李春英 赵雍 王连嵋 李桂琴 |
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| 作者单位： | 中国中医科学院 中药研究所, 中药鉴定与安全性检测评估北京市重点实验室, 北京 100700,中国中医科学院 中药研究所, 中药鉴定与安全性检测评估北京市重点实验室, 北京 100700,中国中医科学院 中药研究所, 中药鉴定与安全性检测评估北京市重点实验室, 北京 100700,中国中医科学院 中药研究所, 中药鉴定与安全性检测评估北京市重点实验室, 北京 100700,中国中医科学院 中药研究所, 中药鉴定与安全性检测评估北京市重点实验室, 北京 100700,中国中医科学院 中药研究所, 中药鉴定与安全性检测评估北京市重点实验室, 北京 100700,中国中医科学院 中药研究所, 中药鉴定与安全性检测评估北京市重点实验室, 北京 100700,中国中医科学院 中药研究所, 中药鉴定与安全性检测评估北京市重点实验室, 北京 100700 |
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| 基金项目： | 国家"重大新药创制"科技重大专项(2015ZX09501004);中国中医科学院博士研究生创新人才培养基金项目 |
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| 摘 要： | 中药遗传毒性研究是中药安全性研究的重要组成部分。我国中药的遗传毒性研究起步较晚,在20世纪70年代以后逐步得到重视。目前发展较为成熟的遗传毒性评价方法主要有以遗传基因突变为指标的试验(细菌回复突变试验、小鼠淋巴瘤tk试验)、以染色体畸变为指标的试验(微核试验、染色体畸变试验)和以DNA损伤为指标的试验(单细胞凝胶电泳检测)3种。 该文结合中药特点就现代中药遗传毒性研究进展、遗传毒性评价方法、中药遗传毒性评价存在的问题及解决方法、新技术在遗传毒性评价中的应用等内容进行综述。
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| 关 键 词： | 中药遗传毒性 遗传毒性评价方法 中药遗传毒性研究的问题和解决方法 遗传毒性评价新技术 |
| 收稿时间： | 2015/3/31 0:00:00 |
Genotoxicity research thought and method on traditional Chinese medicine |
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| HAN Jia-yin,YI Yan,LIANG Ai-hu,ZHANG Yu-shi,LI Chun-ying,ZHAO Yong,WANG Lian-mei and LI Gui-qin.Genotoxicity research thought and method on traditional Chinese medicine[J].China Journal of Chinese Materia Medica,2015,40(14):2696-2700. |
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| Authors: | HAN Jia-yin YI Yan LIANG Ai-hu ZHANG Yu-shi LI Chun-ying ZHAO Yong WANG Lian-mei and LI Gui-qin |
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| Institution: | Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China,Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China,Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China,Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China,Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China,Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China,Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China and Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China |
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| Abstract: | Genotoxicity research takes an important place in traditional Chinese medicine safety evaluation. Genotoxicity test on traditional Chinese medicine has been paid great attention since 1970s. Currently, the most developed genotoxicity test methods included: bacterial reverse mutation test and mouse lymphoma assay which are used to detect relevant genetic changes, micronucleus test and chromosomal analysis which are used to measure chromosomal aberration, and single cell electrophoresis assay which is used to test DNA damage. This article reviews research progress on genotoxicity of traditional Chinese medicine, evaluation methods of genotoxicity, the problems and solutions on genotoxicity evaluation of traditional Chinese medicine, and new technique used in genotoxicity test. |
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| Keywords: | genotoxicity on traditional Chinese medicine genotoxicity test methods problems and solutions on genotoxicity evaluation of traditional Chinese medicine new technique used in genotoxicity test |
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