nm23—H1基因与乳腺癌转移和预后的关系及其突变的研究

采用免疫组化染色分析100例乳腺癌组织的nm23－H1基因表达与腋窝淋巴结转移和预后的关系，发现阳性表达者的5年无瘤生存率为84．8％（39／46），明显高于阴性表达者的52．9％（18／34），P＜0．01。进一步对20例采用RNA提取、PT－PCR、PCR－SSCP等方法检测乳腺癌组织中nm23－H1基因mRNA表达和突变情况，发现有突变的乳腺癌其基因表达均为阴性，胶窝淋巴结转移均为阳性（P＝0．036）。研究结果提示：nm23－H1基因突变可能易发生于晚期乳腺癌，同时检测nm23－H1基因mRNA表达水平和突变情况可作为判断乳腺癌（尤其是无淋巴结转移乳腺癌）的一项指标。

Relationship of nm23-H_1 expression and mutation with the metastasis and prognosis of breast cancer

Immunohistochemical (IHC) staining was used in our study to detect the expression of nm23-H1 gene in 100 specimens of breast cancer, their relationship with metastasis and prognosis was analyzed. The results showed that the five year dissease-free survival rate of patients with positive protein expression was 84. 8 % (39/46), but those with negative expression was 52. 9 % (18/ 34 ). Twenty specimens were further studied for nm23-H1 mRNA expression and gene mutation with RNA isolation,RT-PCR, PCR-SSCP and other methods. The results demonstrated that the mRNA expression and gene mutation were associated with their protein expression. nm23-H1 mutation was also associated with lymph node metastasis. The authors suggest that mutation and deletion or abnormal RNA expression of nm23-H1 gene might be the causes of its negative protein expression,and could be useful in evaluating prognosis of breast cancer patients.