| 棘阿米巴土壤分离株CB/S1的18S rDNA基因型鉴定 |
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| 引用本文： | 郑善子,玄英花,王月华.棘阿米巴土壤分离株CB/S1的18S rDNA基因型鉴定[J].中国人兽共患病杂志,2006,22(11):1039-1042. |
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| 作者姓名： | 郑善子 玄英花 王月华 |
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| 作者单位： | [1]延边大学基础医学院免疫学与病原生物学教研部,延吉133000 [2]吉林医药学院病原生物学教研室,延吉133000 |
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| 摘 要： | 目的鉴定从北京市区土壤中分离的棘阿米巴分离株Acanthamoeba sp．CB／S1的18S rDNA基因型。方法从土壤分离的CB／S1株中提取基因组18SrDNA，应用棘阿米巴属特异性引物PCR扩增18S rDNA序列。将扩增产物测序后用分子生物学软件Clustal X进行序列分析，与基因库中已有T1至T12型序列进行比较并构建进化树。结果与结论从北京市区土壤中分离的的棘阿米巴分离株CB／S1的18S rDNA全基因序列分别为2291bp，其基因型属于T5型。
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| 关 键 词： | 棘阿米巴 基因型 分类 |
| 文章编号： | 1002-2694(2006)11-1039-03 |
| 修稿时间： | 2006年4月16日 |
Genotype identification of 18S rDNA from Acanthamoeba sp. CB/S1 isolated from soil in Beijing |
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| ZHENG Shan-zi,XUAN Ying-hua,WANG Yue-hua.Genotype identification of 18S rDNA from Acanthamoeba sp. CB/S1 isolated from soil in Beijing[J].Chinese Journal of Zoonoses,2006,22(11):1039-1042. |
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| Authors: | ZHENG Shan-zi XUAN Ying-hua WANG Yue-hua |
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| Abstract: | The genomic 18S rDNA sequence from Acanthamoeba sp. CB/S1 isolated from soil in Beijing was extracted, and was amplified by PCR using the Acanthamoeba sp specific primers designed. The amplified product was undergone with sequence analysis by molecular biological software Clustal X and compared with the T1 to T12 types of GenBank, thus establishing the phylogenetic tree. It was found that the full length of the CB/S1 gene was 2291 bp and the genotype of the strain CB/S1 was proved to be T5 type. |
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| Keywords: | 18S rDNA |
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