Predictive potential of glutathione S-transferase polymorphisms for prognosis of osteosarcoma patients on chemotherapy

Objective: To evaluate the predictive value of glutathione S-transferase (GST) gene polymorphisms for theprognosis of osteosarcoma patients receiving chemotherapy. Methods: A total of 159 patients were included inour study between January 2005 and December 2007., with follow-up until January 2012. Genotyping was basedupon the duplex polymerase-chain-reaction with the PCR-CTPP method. Results: At the time of diagnosis, 15.4%of the patients presented with metastasis, while 22.3% developed metastasis during follow-up. At the time of finalanalysis on January 2012, the median follow-up was 45.5 months. Patients with null GSTM1 and GSTT1 hada higher event free survival rate than non-null genotype, but no significant association was found between thetwo genotypes and prognosis of osteosarcoma. Individuals with GSTP1 Val/Val genotype tended to live shorterthan with the IIe/IIe genotype, and we found a significantly higher risk of death from osteosarcoma (adjustedHR=2.35, 95% CI=1.13-4.85). Conclusion: The GSTP1 gene polymorphism may have an important role in theprognosis of osteosarcoma patients with chemotherapy. Further analyses with larger samples and more genesencoding metabolizing and DNA repair enzymes are warranted.