电针对雌雄两性实验性肥胖大鼠脂代谢的影响

目的:探讨电针刺激对雌雄两性肥胖大鼠脂代谢影响的差异及其作用机制。方法:SD大鼠随机分为模型雌组、模型雄组、电针雌组、电针雄组,并设正常雌组和正常雄组进行对照。采用谷氨酸钠和高脂饮食诱导的下丘脑性肥胖模型。电针雌组、雄组电针大鼠"后三里"三阴交"丰隆"等穴。计算各组治疗前后Lee’s指数,生化法检测各组血浆甘油三酯(TG)、胆固醇(TC)、低密度脂蛋白(LDL-C)、高密度脂蛋白(HDL-C)含量。结果:电针雌、雄两组的Lee’s指数及血浆TG、TC、LDL-C含量均较模型雌、雄两组明显降低(P<0.01,P<0.05),而血浆HDL-C含量均有明显升高(P<0.05)。电针雄组Lee’s指数较电针雌组明显降低(P<0.05);电针雌组血浆TG、TC含量较电针雄组明显降低(P<0.05);电针雌组血浆HDL-C、LDL-C的含量与电针雄组比较差异无统计学意义(P>0.05)。结论:电针刺激对雌雄两性肥胖性大鼠均有不同程度的减肥作用,但在降低Lee’s指数和调节脂代谢方面存在着一定的差异,电针可更明显地降低雄性大鼠的Lee’s指数及雌性大鼠的血浆TG、TC含量,但对雌雄两性血浆HDL-C、LDL-C含量的影响无明显差异。

Effect of electroacupuncture on lipid metabolism in male and female obesity rats

Objective To investigate the influence of electroacupuncture(EA) on lipid metabolism in both sexes of obesity model rats.Methods A total of 30 male and 30 female SD rats were randomized into normal control,model and EA groups respectively,with 10 rats in each group.EA(2 Hz,1-2 mA) was applied to the ipsilateral "Zusanli"(ST 36)-"Sanyinjiao"(SP 6) and bilateral "Fenglong"(ST 40) for 30 min,once daily for 28 days.In addition,"Quchi"(LI 11),"Zhongwan"(CV 12)and"Guanyuan"(CV 4) were punctured with filiform needles and stimulated by manipulating the needle for 1 min,separately.Obesity model was duplicated by subcutaneous injection of 15% glutamic sodium(0.2 mL/10 g) once daily for 5 days and by feeding the animal with high fat forage for 36 days.Lee's index was calculated,and plasma triglyeride(TG) concentration detected by glycerol-phosphoric acid oxidase-peroxydase method,total cholesterol(TC) detected by cholesterol oxidase method,and plasma low-density lipoprotein-cholesterol(LDL-C) and high-density lipoprotein-cholesterol(HDL-C) contents were assayed by one-step method,respectively.Results In both male and female rats,compared to their own normal groups,the Lee's index,plasma TG,TC and LDL-C contents were increased significantly and plasma HDL-C contents decreased obviously in the model groups(P<0.01),while compared to their own model groups,the Lee's index,plasma TG,TC and LDL-C levels in the EA groups were down-regulated considerably and plasma HDL-C contents up-regulated remarkably after the treatment(P<0.01,P<0.05).The efficacies of the EA intervention were obviously better in reducing Lee's index for male rats,and in down-regulating plasma TG and TC contents in female rats(P<0.05).No significant differences were found between male and female rats in EA intervention for plasma LDL-C and HDL-C levels.Conclusion EA intervention can effectively regulate obesity rats' Lee's index and plasma TG,TC,LDL-C and HDL-C levels.The effects are different in male and female rats.