荷瘤鼠及口腔癌患者热化疗前后T淋巴细胞亚群、白细胞介素-2和肿瘤坏死因子-α水平的变化

目的探讨荷瘤鼠及口腔癌患者热化疗前后T淋巴细胞亚群、白细胞介素（IL）-2和肿瘤坏死因子（TNF）-α活性水平的变化,并初步探讨三者之间的相关性。方法经热化疗处理后,采用甲基噻唑基四唑（MTT）法检测荷瘤鼠淋巴细胞转化指数（LTI）及IL-2、TNF-α水平,MTT法检测口腔癌患者IL-2、TNF-α水平,并采用3H-胸腺嘧啶核苷（3H-TdR）掺入法行淋巴细胞转化实验及CD4+、CD8+ T细胞亚群检测。结果高温联合平阳霉素治疗组（HP组）荷瘤鼠LTI、IL-2和TNF-α活性与正常对照组（N组）荷瘤鼠差异无统计学意义（P>0.05）,而明显高于平阳霉素治疗组（P组）及不治疗组（NT组）（P<0.01）。临床实验中热化疗后口腔癌患者LTI、CD4+细胞数目及CD4+/CD8+比值明显提高,IL-2和TNF-α活性水平明显升高（P<0.01）。结论荷瘤宿主的LTI及IL-2和TNF-α活性水平在热化疗后明显升高,IL-2和T细胞之间有明显的相关性。

Change of T lymphocyte subsets, interleukin-2 and tumor necrosis factor-alpha in tumor-bearing mice and patients with oral cancer receiving thermo-chemotherapy

Objective To investigate the change of T lymphocyte subsets, interleukin（IL）-2 and tumor necrosis factor（TNF）-α in the tumor- bearing mice and patients with oral cancer receiving thermo-chemotherapy, and investigate the correlation among them. Methods After treatments, the expression of lymphocyte transformation index（LTI）, IL-2 and TNF-α in the tumor-bearing mice were detected with methyl thiazolyl tetrazolium（MTT）, the expression of IL-2 and TNF-α in the patients with oral cancer were detected with MTT, the expression of LTI, CD4+ and CD8+ were detected with 3H-TdR incorporation. Results LTI, IL-2 and TNF-α of thermo-chemotherapy group（HP group） had no significant difference comparing with those of normal mice group（N group）（P>0.05）, but which were significantly
higher than those of chemotherapy group（P group） and no treatment group（NT group）（P<0.01）. In clinical trials, the expression of LTI, CD4+, CD4+/CD8+, IL-2 and TNF-α on oral cancer patients after thermo-chemotherapy were significantly higher than those before thermo-chemotherapy（P<0.01）. Conclusion After thermo-chemotherapy, the expression of LTI, IL-2 and TNF-α of tumor-bearing hosts are significantly improved, there is a significant correlation between IL-2 and T cell.