八桂止痛膏对小鼠镇痛 抗炎作用效果实验研究

目的探讨八桂止痛膏(BAO)对小鼠镇痛、抗炎作用效果。方法选择昆明种雌性小鼠作为实验对象,根据干预方式不同将其分为空白对照组(不予药物干预)、扶他林组(予扶他林软膏干预)和BAO组(予BAO干预)。通过热板实验、醋酸扭体实验和二甲苯致小鼠耳廓肿胀实验评估干预处理对小鼠的镇痛、抗炎效果。结果在给药后15～45 min,扶他林组痛阈值呈逐渐上升趋势,而BAO组痛阈值呈先上升后下降趋势。两组给药后各时间点的痛阈值均高于空白对照组,差异有统计学意义(P <0.05)。在给药后45 min时间点,BAO组痛阈值低于扶他林组,差异有统计学意义(P <0.05)。扶他林组和BAO组扭体次数低于空白对照组,差异有统计学意义(P <0.05)。扶他林组和BAO组扭体次数和扭体次数抑制率比较差异无统计学意义(P> 0.05)。扶他林组耳廓肿胀质量小于BAO组和空白对照组,BAO组耳廓肿胀质量亦小于空白对照组,差异有统计学意义(P <0.05)。结论 BAO具有较好的镇痛、抗炎作用,其效果与扶他林软膏相当。

An experimental study on the analgesic and anti-inflammatory effects of Bagui Analgesic Ointment on mice

Objective To explore the analgesic and anti-inflammatory effects of Bagui Analgesic Ointment(BAO)on mice.Methods Kunming female mice were selected as the experimental subjects,and they were divided into blank control group(no drug intervention),Voltaren group(receiving Voltaren ointment intervention)and BAO group(receiving BAO intervention)according to different intervention methods.The analgesic and anti-inflammatory effects of the intervention treatments on mice were evaluated by the hot plate experiment,the acetic-acid-induced twisting experiment and the xylene-induced auricle swelling experiment in mice.Results From 15 minutes to 45 minutes after administration,the pain threshold of Voltaren group showed a gradually increasing trend,while the pain threshold of BAO group showed an increasing trend at first and then a decreasing trend.The pain thresholds of the two groups at each time point after administration were higher than those of the blank control group,and the differences were statistically significant(P<0.05).At the time point of 45 minutes after administration,the pain threshold of BAO group was lower than that of the Voltaren group,and the difference was statistically significant(P<0.05).Both the Voltaren group and the BAO group had lower number of body twisting than the blank control group,and the differences were statistically significant(P<0.05).There were no significant differences in the number of body twisting and the inhibition rate of body twisting between the Voltaren group and the BAO group(P>0.05).The mass of the swollen auricles in the Voltaren group was smaller than that in the BAO group and the blank control group,and the mass of the swollen auricles in the BAO group was also smaller than that in the blank control group,and the differences were statistically significant(P<0.05).Conclusion BAO has better analgesic and anti-inflammatory effects,and its effects are equivalent to those of Voltaren ointment.