邻苯三酚—鲁米诺发光体系测定SOD活性

目的：建立一种测定ＳＯＤ活力的方法。方法：利用邻苯三酚在碱性条件下自氧化—鲁米诺化学发光体系测ＳＯＤ活性。结果：本方法一个活力单位（抑制５０％发光程度）的ＳＯＤ量为１６ｎｇ／ｍｌ。本法测定ＳＯＤ浓度范围为５～１５０ｎｇ／ｍｌ，线性回归方程的相关系数：ｒ＝０．９９８。检测限为１ｎｇ。重复性：批内Ｃ．Ｖ．＝１．０％，批间（隔７ｄ）Ｃ．Ｖ．＝５．１％。结论：本法具有简易、灵敏、试剂价廉等优点，特别适宜于植物样品的ＳＯＤ活力测定

Chemiluminescence Assayfor SOD Activity Using Alkaline Pyrogallol as Superoxide Anion-Generating System

Objective: This article is to develop a simple sensitive method of SOD assay. Methods: The SOD activity was measured with the luminol chemiluminescence of superoxide anion generated by the autoxidation of alkaline pyrogallol. Results: Fifty percent inhibition,corresponding to one unit of the enzyme,was produced by 16 ng of pure SOD.SOD was determined in the range of 5150 ng with a correlation coefficient of 0.998 for the linear regression equation by transforming SOD value into logarithms and a limit of detection of 1 ng of SOD.The coefficient of variance is 1.0%for the sample from an assay and 5.1%for the sample from an individual estimated at two different times (over a one-week interval). Conclusion: This method is simple,sensitive and cheap.It is especially appropriate for the SOD assay of botanical samples.