溴氰菊酯和辛硫磷亚致死剂量对德国小蠊酶活性的影响

目的研究溴氰菊酯和辛硫磷亚致死剂量处理后德国小蠊羧酸酯酶、谷胱甘肽S-转移酶、乙酰胆碱酯酶的活性变化规律。方法采用分光光度计离体测定酶的活性，应用DPS软件进行统计分析。结果辛硫磷亚致死剂量处理德国小蠊后24h，羧酸酯酶活性降到最低，与处理前相比降低了79．33％，24h后其活性逐渐恢复，168h达到处理前的37．10％；溴氰菊酯亚致死剂量处理后，羧酸酯酶活性略有下降；经溴氰菊酯和辛硫磷亚致死剂量处理后，谷胱甘肽S-转移酶的活性略有下降，72h后其活性分别为处理前的74．97％和79．77％；经溴氰菊酯亚致死剂量处理后，乙酰胆碱酯酶活性24h略有升高，随后逐渐下降，经辛硫磷处理后其活性略有降低。结论经溴氰菊酯和辛硫磷亚致死剂量处理后德国小蠊体内酶活性在不同的时间与处理前存在差异。

Effect of sublethal doses of deltamethrin and phoxim on enzyme activity of Blattella germanica

Objective Activities of carboxylesterase, glutathione S-transferase and acetylcholinesterase from German cockroach (Blattella germanica) treated with sublethal doses of deltamethrin and phoxim were measured. Methods Enzyme activities were measured in vitro by spectrophotometry and DPS was used for statistical analysis. Results Activity of carboxylesterase reduced to the lowest level at 24 h after treated with sublethal doses of phoxim. The activity decreased 79.33% compared with that before treatment, then recovered gradually and at 168 h reached 37.10% of that of before treatment. Activity of carboxylesterase reduced slightly after treated with sublethal doses of deltamethrin. For glutathione Stransferase, activities declined slightly and were 74.97% and 79.77% after treatment for 72 h , respectively. Activity of acetylcholinesterase increased within 24 h after treated with sublethal doses of dehamethrin, then declined gradually. Activity reduced slightly after treated with sublethal doses of phoxim. Conclusion Compared with before treatment, the enzyme activity of German cockroach changes with the difference of time after treated with sub-lethal doses of deltamethrin and phoxim.