孕激素受体亚型在子宫肌瘤组织中的表达

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Protein expression of human progesterone receptor isoforms A and B in uterine leiomyoma

OBJECTIVE: To investigate the role of two isoforms of human progesterone receptor A (hPR-A) and B (hPR-B) in the development of uterine leiomyoma, their distribution and expression in leiomyoma were detected. METHODS: The tissues of leiomyoma and normal myometrium from 30 uteri, which were excised for leiomyoma, were used for the localization and quantification of protein of the two isoforms. Immunohistochemistry and Western blot were applied respectively. RESULTS: Both hPR-A and hPR-B were nuclear receptors. Concentrations of hPR-(A + B) and hPR-A in leiomyoma were higher than those in normal myometrium (295,796 +/- 90,856, 256,275 +/- 98,560; P = 0.042, P = 0.000,563). Both isoforms were presented in leiomyoma and normal myometrium, with a consistent dominance of hPR-B over hPR-A. More expression of hPR-A was found during secretive phase than proliferative phase, not only in leiomyoma but also in normal myometrium (P = 0.037, P = 0.024). CONCLUSIONS: The development of uterine leiomyoma seems to be related with the progesterone receptor isoforms, especially hPR-A.