抗衰老片对肾虚小鼠空间学习记忆和脑自由基代谢的影响

目的观察抗衰老片对肾虚小鼠空间学习记忆和脑自由基代谢的影响。方法选雄性KM小鼠54只，随机分为6组：正常对照组、肾虚组（劳倦过度＋房事不节组）、抗衰老片高、中、低剂量组和补肾方（金匮肾气丸）对照组。采用强迫小鼠游泳方法造成劳倦过度，以Colldege效应诱导雄性小鼠房事不节，建立肾虚小鼠模型，观察小鼠体重、水迷宫空间行为记忆能力和脑组织SOD活性和MDA含量变化。结果肾虚组小鼠体重、脑组织SOD活性较正常对照组明显下降俨〈0．05，P〈0．01），小鼠水迷宫潜伏期和脑组织MDA含量明显升高（P〈0．01）；抗衰老片高、中、低剂量组及金匮肾气丸组小鼠体重和脑组织SOD活性较肾虚组明显升高伊〈0．05，P〈0．01），小鼠水迷宫潜伏期和脑组织MDA含量较肾虚组明显降低（P〈0．05，P〈0．01）。结论抗衰老片可延缓脑组织的脂质过氧化，从而改善小鼠的记忆能力。

Effect of Anti-aging Tablets on Special Learning Memory and Free Radical Metabolism in Brain of Kidney Deficiency Mouse

Objective To observe the effects of Anti-aging tablets on special learning memory and free radical metabolism in brain of kidney deficiency mouse. Methods Fifty-four male KM mice were categorized into six groups randomly： normal control group, kidney deficiency group （over-fatigue plus sexual overstrain group）, low, middle, high doses of Anti-aging tablets groups and Golden Chamber Pill group. Over-fatigue model could be made by forcing mice to swim and kidney deficiency by colldege effect to induce sexual overstrain in male mice. Anti-aging tablets and Golden Chamber Pill could serve as medication. Changes of weight, water maze special learning and memory test, SOD activity and MDA content of brain were examined in different groups. Results The weight and SOD activity of brain in kidney deficiency group were lower than those in control groups （P〈0.05, P〈0.01）, and the latency period of water maze and MDA content of brain were higher than those in control groups （P〈0.01）, while the weight and SOD activity of brain were higher in Anti-aging tablets groups and Golden Chamber Pill group （P〈0.05, P〈0.01）, and the latency period of water maze and MDA content of brain were lower in Anti-aging tablets groups and Golden Chamber Pill group （P〈0.05, P〈0.01）, compared with those in kidney deficiency group. Conclusion The Anti-aging tablets could be increased the activity of antioxirant enzymes and suppressed the generation of lipid peroxides, and which could be improved the memory ability of the mice.