ICAM-1基因多态性与HBV感染不同临床结局之间的关系

目的探讨细胞间黏附分子l（ICAM-1）基因多态性与HBV感染不同临床结局之间的相关性。方法应用病例．对照研究和聚合酶链反应-序列特异性引物法（PCR-SSP）检测118例慢性持续性HBV感染患者（包括无症状HBV携带者、慢性乙型肝炎、乙肝后肝硬化患者）和60例HBV急性自限性感染者的ICAM-1基因G241R（G／A）、K469E（A／G）两个位点的多态性，比较各组间基因型和等位基因频率，并对数据进行统计分析。结果①ICAM-l G241R（G／A）位点总GG基因型频率在HBV慢性持续性感染组高于急性自限性感染组，但差异无统计学意义（X^2=1．38，P〉0．05）。②ICAM-1 K469E（A／G）位点，进展性肝病组（慢性乙型肝炎和肝硬化）总KK基因型和总K等位基因的频率与无症状携带者组和自限性感染组相比显著增高（X^2=8．60，P〈0．05；X^2=5．07，P〈0、05），而在自限性感染和无症状携带者之间却无显著差异。结论携带ICAM-1 K469E KK基因型和K等位基因的患者容易进展成慢性乙型肝炎甚至肝硬化，可致慢性HBV感染患者病情进展。

Association of ICAM-1 gene polymorphism with clinical outcomes of Hepatitis B virus infection

Objective To investigate the associations of ICAM-1 gene polymorphism with the clinical outcomes of HBV infection. Methods A case-control study and PCR-SSP were used to detect ICAM-1 gene G241R and K469E pol- ymorphism of 118 chronic HBV infected patients and 60 subjects spontaneously recovered from HBV infection（ SR group）. The chronic hepatitis B infection group included 36 asymptomatic HBV carriers（AsC group） ,50 chronic hepatitis B and 32 HBV-related liver cirrhosis. Results The frequency of the GG genotype in G241R was higher in chronic HBV infection group than that in SR group, but it （ P 〉 0.05 ） had no statistic significance. The frequency of the K allele and KK genotype in K469E was significantly higher in all advanced active liver disease group（ including chronic hepatitis B and liver cirrhosis）than that in SR or AsC group（X^@ =8.60,P 〈0.05;X^2=5.07,P 〈0.05）. Conclusion ICAM-1 Kd69E KK genotype and K allele may be associated with susceptibility of chronic hepatitis B, even liver cirrhosis.