| 高效液相色谱分离纯化鼠疫菌Pla蛋白 |
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| 引用本文： | 石丽媛,杨光璨,董珊珊,于国林.高效液相色谱分离纯化鼠疫菌Pla蛋白[J].中国地方病防治杂志,2009(2):102-106. |
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| 作者姓名： | 石丽媛 杨光璨 董珊珊 于国林 |
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| 作者单位： | 云南省地方病防治所中心实验室; |
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| 摘 要： | 目的用高效液相色谱分离纯化鼠疫菌纤溶酶原激活因子。方法优化离子交换、凝胶过滤，并将2种层析组合实现纯化。结果用高效液相色谱纯化的Pla主要由相对分子质量约31×10^3、35×10^3、37×10^3的3条蛋白带组成，占蛋白总量80％以上。结论Pla经高效液相色谱分离可以达到基本纯化。
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| 关 键 词： | 高效液相色谱 耶尔森菌 鼠疫 纤溶酶原激活因子 |
Purification of Plasminogen activator of Yersinia pestis by HPLC |
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| SHI Li-yuan,YANG Guang-can,DONG Shan-shan,et al.Purification of Plasminogen activator of Yersinia pestis by HPLC[J].Chinese Journal of Control of Endemic Disenaces,2009(2):102-106. |
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| Authors: | SHI Li-yuan YANG Guang-can DONG Shan-shan |
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| Institution: | Yunnan Institute of Endemic Diseases Control and Prevention;Dali 671000 |
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| Abstract: | Objective To purify Plasminogen activator of Yersinia pestis by high performance liquid chromatography （ HPLC）. Methods We optimized gel filtration and ion exchange, and combined them to purify Pla. Results Pla purified by HPLC was mainly composed of three bands （ Mr about 31 ×10^3 ,35 ×10^3 ,37×10^3） , occupying more than 80 percent. Conclusions Pla is purified basically by HPLC. |
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| Keywords: | HPLC Yersinia pestis Plasminogen activator |
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