Visualisation of zebrafish infection by GFP-labelled Vibrio anguillarum |
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| Authors: | O'Toole Ronan Von Hofsten Jonas Rosqvist Roland Olsson Per-Erik Wolf-Watz Hans |
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| Institution: | 1. Department of Molecular Biology, Umeå University, S-901 87 Umeå, Sweden;2. School of Biological Sciences, Victoria University of Wellington, PO Box 600, Wellington, New Zealand;3. Department of Natural Sciences, Örebro University, S-701 82, Örebro, Sweden;1. Yale New Haven Hospital, New Haven, CT, USA;2. The University of Vermont College of Medicine, Burlington, VT, USA;3. Fletcher Allen Health Care, Burlington, VT, USA;1. Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;2. Functional Laboratory of Marine Fisheries Science and Food Production Process, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China;3. Liaoning Key Laboratory of Marine Animal Immunology & Disease Control, Dalian Ocean University, Dalian 116023, China;4. University of Chinese Academy of Sciences, Beijing 100049, China;1. Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China;2. Key Laboratory of Mariculture & Stock Enhancement in North China’s Sea, Ministry of Agriculture, Dalian Ocean University, Dalian, 116023, China;3. University of Chinese Academy of Sciences, Beijing, 100049, China |
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| Abstract: | Vibrio anguillarum is an invasive pathogen of fish causing a septicaemia called vibriosis. In this work, transparent zebrafish were immersed in water containing green fluorescent protein labelled V. anguillarum. The infection was visualised at the whole fish and single bacterium levels using microscopy. The gastrointestinal tract was the first site where the pathogen was detected. This enteric localisation occurred independently of the flagellum or motility. On the other hand, chemotactic motility was essential for association of the pathogen with the fish surface. In conclusion, the zebrafish infection model provides evidence that the intestine and skin represent sites of infection by V. anguillarum and suggests a host site where chemotaxis may function in virulence. |
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