印迹基因H19在早孕绒毛组织中印迹状态的研究

目的检测早孕绒毛组织中印迹基因H19印迹的状态,探讨其印迹状态与滋养细胞浸润能力之间的联系。方法PCR-RFLP方法检测93例正常早孕绒毛组织中H19的印迹状态。结果在93例正常早孕绒毛组织中,有42例为杂合子,杂合子基因型中有11例为双等位基因的表达,其中所有双等位基因表达均在孕5～9周,而孕10～12周未发现有双等位基因的表达。结论在早孕期间,H19基因在绒毛组织中存在双等位基因的表达,主要集中在孕10周前,提示H19基因在滋养细胞中可能存在有动态变化,并与滋养细胞的浸润能力的变化有一定的联系。

Imprinting Status of H19 Gene in Normal Villi Tissue During First Trimester: A Preliminary Study

OBJECTIVE To study the imprinting status of H19 gene in normal villi tissue during the first trimester,and its relation to the invasion of trophoblast. METHODS Using PCR-RFLP methods to examine the imprinting status of H19 gene in 93 cases of normal villi tissue during the first trimester. RESULTS Among 93 cases, heterozygous genotypes were found in 42 cases. And 11 cases of biallelic expression were found among these 42 cases of heterozygous genotypes from 5 to 9 weeks, however no biallelic expression existed from 10 to 12 weeks. CONCLUSIONS During the first trimester, H19 is expressed biallelically at the first 10 weeks. The H19 gene may dynamicly change in the trophoblast, and the dynamic change may have close relationship with the invasion of the trophoblast.