| 侧脑室注射重组人血管生长素对脑缺血再灌注大鼠的影响 |
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| 引用本文： | 张伟赫,郭淮莲,程敏,唐文雄,唐春花,高旭光.侧脑室注射重组人血管生长素对脑缺血再灌注大鼠的影响[J].中风与神经疾病杂志,2010,27(2). |
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| 作者姓名： | 张伟赫 郭淮莲 程敏 唐文雄 唐春花 高旭光 |
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| 作者单位： | 北京大学人民医院神经内科,北京,100044 |
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| 基金项目： | 国家自然科学基金资助项目(No.30670733) |
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| 摘 要： | 目的观察经大鼠侧脑室注射重组人血管生长素(Angiogen in,ANG)对脑缺血再灌注大鼠的影响。方法线栓法制备大鼠脑缺血再灌注模型,大鼠随机分成4组:ANG治疗组、牛血清白蛋白对照组、MCAO对照组及假手术组,观察大鼠体重的变化,并于治疗3d后进行HE染色、细胞凋亡检测以及vWF因子免疫组织化学染色。结果脑缺血再灌注3d后ANG治疗组大鼠与MCAO对照组及牛血清白蛋白对照组相比,体重显著降低(P<0.05),缺血脑区神经元变性、坏死、间质水肿以及胶质细胞增生程度减轻,凋亡阳性细胞数明显减少(P<0.05),微血管计数增加(P<0.05)。结论侧脑室注射ANG可改善脑缺血大鼠脑内间质水肿和神经元变性,降低凋亡细胞数量,增加微血管数量。ANG可降低脑缺血大鼠体重。
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| 关 键 词： | 侧脑室注射 血管生长素 脑缺血再灌注 |
The effect of intraventricular administration of recombinant human angiogenin on cerebral ischemia-reperfusion rat |
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| ZHANG Wei-he,GUO Huai-lian,CHENG Min,et al..The effect of intraventricular administration of recombinant human angiogenin on cerebral ischemia-reperfusion rat[J].Journal of Apoplexy and Nervous Diseases,2010,27(2). |
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| Authors: | ZHANG Wei-he GUO Huai-lian CHENG Min |
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| Institution: | ZHANG Wei-he,GUO Huai-lian,CHENG Min,et al.(Department of Neurology,People\'s Hospital,Peking University,Beijing 100044,China) |
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| Abstract: | Objective To observe the effect of intraventricular administration of recombinant human angiogenin(ANG) on rats that suffered from cerebral ischemia-reperfusion.Method Cerebral ischemia-reperfusion model was established by modified Longa's method,and the rats were randomly divided into four groups: ANG group,bovine serum albumin group,MCAO group and Sham.We observed their weights,and carried out hematoxylin eosin staining,TUNEL staining,and immunohistochemical staining for vWF on 3 days after ischemia-reper... |
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| Keywords: | Intraventricular injection Angiogenin Brain ischemia-reperfusion |
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