西藏土壤厌氧芽胞梭菌的调查分析

目的　了解西藏高原土壤厌氧芽胞细菌的种类、数量及其对药物敏感情况 ,为平战时感染提供诊疗依据。方法　按照Bergey’s手册对土样进行厌氧培养分离及生化反应鉴定。结果　每克土壤中厌氧芽胞梭菌数为 :30～ 1 6 2 0 0 0、细菌总数为 :0 .1 1× 1 0 7～ 88.6× 1 0 7;同一地区不同地点、同一地点不同深度细菌数和梭菌数相差甚大。未开垦地区不能培养出厌氧细菌 ,75cm以下不能检出厌氧细菌。分离出的 2 7种 93株厌氧芽胞梭菌中主要为产气荚膜梭菌占 2 0 4 %。结论　西藏地区土壤存在有能产生气性坏疽的厌氧芽胞梭菌及其它厌氧菌 ,但大部分来自人或动物的粪便 ,其对常用抗生素的敏感率均在 90 %以上

Investigation of Soil Anaerobic Sporeforing Bacilli in Tibet High Altitude

Objective To investigate the situation of soil anaerobic sporeforing bacilli in Tibet high altitude including its class, number and the sensibility to drugs, which may help diagnosis and treatment of infection in peace and war time. Methods According to Bergey's manual, bacteria from soil sample were cultured in anoxic condition, isolated and differentiated by biochemistry reaction. Results The number of anaerobic sporeforing bacilli in per gram soil was 30 and 162 000. The number of all kinds of bacteria was 0.11×10 7～88.6×10 7 per gram. There were great differences in bacterial numbers of different points and different depths soil in the same area. There was no anaerobic sporeforing bacilli be found in unsorted land and soil deeper than 75 cm. In all isolated 27 classes, 96 strains of bacterium, Clotridium per fringens accounts for 20.4 percent. Conclusion There were anaerobic sporeforing bacilli and other anaerobes responsible for gas gangrene in Tibet soil, which mainly come from feces of mankind and animals. The sensitive rates to common antibiotics fo these bacteria were all above 90 percent.