Synergistic effects of pemetrexed and amrubicin in non-small cell lung cancer cell lines: Potential for combination therapy |
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Authors: | Yukihisa Hatakeyama Kazuyuki KobayashiTatsuya Nagano Daisuke TamuraMasatsugu Yamamoto Motoko TachiharaYoshikazu Kotani Yoshihiro Nishimura |
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Affiliation: | Division of Respiratory Medicine, Department of Internal Medicine, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan |
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Abstract: | The purpose is to examine the synergistic effect of pemetrexed (PEM) and amrubicin (AMR) on the proliferation of lung cancer cell lines. In vitro, dose-dependent synergistic effects of concurrent PEM and AMRol, which is an active metabolite of AMR were observed in A549 and H460 cells. In real-time RT-qPCR analysis and western blotting, expression of the target enzymes of PEM were suppressed in cells treated with amrubicinol alone. In vivo, AMR/PEM treatment also showed synergistic antitumor activity both in A549-bearing and H520-bearing mice. PEM and AMR work synergistically to inhibit the proliferation of several different lung cancer cell lines. |
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Keywords: | Non-small cell lung cancer Thymidylate synthase Pemetrexed Amrubicin |
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