针刺对前列腺增生大鼠防治作用的研究

目的:探讨针刺对前列腺增生的影响及其作用机理。方法:SD雄性大鼠30只,随机分为假手术组、模型组和捻转补法组。皮下注射丙酸睾酮复制前列腺增生大鼠模型。捻转补法组采用捻转针刺补法进行治疗,取“关元”“气海”和“三阴交”穴,留针20 min,每日1次,共针刺21 d。造模及治疗结束后,计算大鼠前列腺重量指数,放免法检测大鼠血清睾丸酮、雌二醇,光镜和电镜下观察大鼠前列腺形态学的变化。结果:模型组大鼠前列腺重量指数和血清雌二醇与假手术组比较明显升高(P<0.01),腺上皮细胞数目与假手术组比较明显增加。针刺组大鼠前列腺重量指数和血清雌二醇与模型组比较明显降低(P<0.01),腺上皮细胞数目与模型组比较明显减少。血清睾丸酮在各组均无明显变化。结论:捻转针刺补法能明显降低前列腺增生大鼠血清雌二醇和前列腺重量指数,从而发挥其抑制前列腺增生的作用。

Study on the Efficacy of Acupuncture in the Treatment and Prevention of Prostate Hyperplasia

Objective: To explore the mechanism of acupuncture in the treatment of prostate hyperplasia in rats.Methods: Thirty SD male rats were randomized into control(sham-operation),model and acupuncture groups,with 10 cases in each group.Prostate hyperplasia model was established by castration under anesthesia(25% Pentobarbital Sodium,(2 mL/kg),i.p.),and subcutaneous injection of Testosterone Propionate((4 mg/kg/d)) for 30 days.The rats in control group were treated with surgical isolation and subcutaneous injection of normal saline."Guanyuan"(CV 4),bilateral "Qihai"(CV 6) and bilateral "Sanyinjiao"(SP 6) were punctured and stimulated by manipulating the needle with twirling reinforcing method.The treatment was conducted once daily,21 days altogether.The prostate weight index (prostate damp weight/body weight)×100%],serum testosterone(Ts),estradiol(E_2) contents were detected with radioimmunoassay,and the anatomical structure of the prostate was observed under light microscope and electronic microscope.Results: Compared with control group,the prostate damp weight,prostate weight index,serum E_2 content and E_2/Ts of model group increased significantly(P<0.05),but the body weight of model group decreased considerably(P<0.05);while compared with model group,the prostate damp weight,prostate weight index,serum E_2 content and E_2/Ts of acupuncture group reduced apparently(P<0.05).No significant differences were found between model and acupuncture groups in body weight,and among the 3 groups in serum Ts level(P>0.05).In model group,the glandular cells arranged in disorder,the lumen of gland enlarged,and the epithelial cells increased in number,while the state of acupuncture group was much better.Conclusion: Acupuncture can significantly restrain prostate hyperplasia,lower serum E_2 level and prostate index in prostate hyperplasia rats.