Quercetin 3-O-methyl ether protects FL83B cells from copper induced oxidative stress through the PI3K/Akt and MAPK/Erk pathway |
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Authors: | Hsiao-Ling Tseng Chia-Jung Li Lin-Huang Huang Chun-Yao Chen Chun-Hao Tsai Chun-Nan Lin Hsue-Yin Hsu |
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Institution: | a Department of Life Sciences, Tzu Chi University, Hualien, Taiwanb Institute of Medical Sciences, Tzu Chi University, Hualien, Taiwanc School of Medicine, Institute of Traditional Medicine, National Yang-Ming University, Taipei, Taiwand Faculty of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwane Department of Biological Science and Technology, School of Medicine, China Medical University, Taichung, Taiwan |
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Abstract: | Quercetin is a bioflavonoid that exhibits several biological functions in vitro and in vivo. Quercetin 3-O-methyl ether (Q3) is a natural product reported to have pharmaceutical activities, including antioxidative and anticancer activities. However, little is known about the mechanism by which it protects cells from oxidative stress. This study was designed to investigate the mechanisms by which Q3 protects against Cu2 +-induced cytotoxicity. Exposure to Cu2 + resulted in the death of mouse liver FL83B cells, characterized by apparent apoptotic features, including DNA fragmentation and increased nuclear condensation. Q3 markedly suppressed Cu2 +-induced apoptosis and mitochondrial dysfunction, characterized by reduced mitochondrial membrane potential, caspase-3 activation, and PARP cleavage, in Cu2 +-exposed cells. The involvement of PI3K, Akt, Erk, FOXO3A, and Mn-superoxide dismutase (MnSOD) was shown to be critical to the survival of Q3-treated FL83B cells. The liver of both larval and adult zebrafish showed severe damage after exposure to Cu2 + at a concentration of 5 μM. Hepatic damage induced by Cu2 + was reduced by cotreatment with Q3. Survival of Cu2 +-exposed larval zebrafish was significantly increased by cotreatment with 15 μM Q3. Our results indicated that Cu2 +-induced apoptosis in FL83B cells occurred via the generation of ROS, upregulation and phosphorylation of Erk, overexpression of 14-3-3, inactivation of Akt, and the downregulation of FOXO3A and MnSOD. Hence, these results also demonstrated that Q3 plays a protective role against oxidative damage in zebrafish liver and remarked the potential of Q3 to be used as an antioxidant for hepatocytes. |
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Keywords: | Quercetin-3-O-methyl ether ROS Cu2 + Erk |
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