透明质酸影响人膝骨关节炎滑膜成纤维样细胞骨桥蛋白和CD44的表达

背景：在骨关节炎病程中,透明质酸水平的改变可以使一系列细胞因子和酶表达水平改变,但是滑膜组织中骨桥蛋白、CD44的高表达是否与透明质酸水平改变相关目前尚不清楚。目的：通过透明质酸干预体外培养的人膝骨关节炎滑膜成纤维样细胞,观察透明质酸对骨关节炎滑膜成纤维样细胞骨桥蛋白和CD44表达的影响。方法：选取接受膝关节镜手术和膝关节置换的原发性膝关节骨关节炎患者滑膜标本10例,进行体外培养获取纯化的滑膜成纤维细胞,取第4~6代滑膜成纤维细胞,分为3组：空白对照组不进行任何干预;透明质酸组用100mg/L透明质酸干预24h;透明质酸酶组用300mg/L透明质酸酶干预24h。结果与结论：与空白对照组相比,透明质酸组滑膜成纤维细胞骨桥蛋白mRNA表达水平上升（P〈0.05）,透明质酸酶组滑膜成纤维细胞骨桥蛋白mRNA表达水平下降（P〈0.05）。透明质酸组和透明质酸酶组之间膜成纤维细胞骨桥蛋白mRNA表达水平差异有显著性意义（P〈0.05）。空白对照组、透明质酸组和透明质酸酶组滑膜成纤维细胞CD44mRNA表达水平差异均无显著性意义（P〉0.05）。提示透明质酸可以使骨关节炎滑膜成纤维细胞骨桥蛋白mRNA表达增高,对CD44mRNA表达没有影响。

Effects of hyaluronic acid on the osteopontin and CD44 expression in fibroblast-like synoviocytes of human with knee osteoarthritis in vitro

BACKGROUND： In the course of osteoarthritis, the changes of hyaluronic acid level can make a series changes of cytokines and enzyme expression levels. However, the relationship between the high expression of osteopontin and CD44 in the synovial tissue and the hyaluronic acid level change is not clear. OBJECTIVE： To change the contents of hyaluronic acid in the fibroblast-like synoviocytes, in order to investigate the effect of hyaluronic acid on the expression of osteopontin and CD44 of fibroblast-like synoviocytes in patients with osteoarthritis of the knee. METHODS： The study was performed on 10 specimens of synovium obtained from osteoarthritic patients. Then all the fibroblast-like synoviocytes 4-6 passaged cells were taken and divided into three groups： the control group without any treatment; hyaluronic acid group was treated with 100 mg/L hyaluronic acid for 24 hours; hyaluronidase group was treated with 300 mg/L hyaluronidase for 24 hours. RESULTS AND CONCLUSION： Compared with control group, the osteopontin mRNA expression in osteoarthritis fibroblast-like synoviocytes was increased in hyaluronic acid group （P 0.05） and decreased in hyaluronidase group （P 0.05）, and the difference of osteopontin mRNA expression showed statistically significance in hyaluronic acid group and hyaluronidase group （P 0.05）. The difference of the CD44 mRNA expression in fibroblast-like synoviocytes was not significant among three groups （P 0.05）. Hyaluronic acid can up-regulate the osteopontin mRNA expression in osteoarthritis fibroblast-like synoviocytes and has no effect on the CD44 mRNA expression.