兔角膜碱烧伤模型房水中肿瘤坏死因子α及血管内皮生长因子表达与姜黄素的抑制

背景：稳定的角膜新生血管动物模型是研究角膜新生血管调控机制,姜黄素对碱烧伤角膜新生血管具有抑制作用和保护作用。目的：探讨姜黄素对碱烧伤角膜新生血管模型中肿瘤坏死因子α及血管内皮生长因子表达的影响,为防治角膜新生血管提供理论依据。方法：纳入33只新西兰大耳白兔,随机取3只设为正常组,其余30只建立兔角膜碱烧伤诱发角膜新生血管模型,右眼设为对照组给予生理盐水,左眼设为干预组给予姜黄素,裂隙灯观察角膜新生血管生长及角膜混浊情况,酶联免疫吸附实验检测肿瘤坏死因子α和血管内皮生长因子在房水中的表达。结果：正常组没有角膜新生血管生成。与对照组比较,干预组角膜新生血管受到抑制且角膜混浊较轻（P〈0.05）。房水肿瘤坏死因子α和血管内皮生长因子在3组中均有表达,对照组和干预组明显高于正常组,但干预组低于对照组（P〈0.05）。说明姜黄素可以有效降低角膜碱烧伤后房水肿瘤坏死因子α及血管内皮生长因子的表达进而抑制兔角膜碱烧伤后角膜新生血管的生长。

Inhibitory effect of curcumin on the expression of vascular endothelial growth factor and tumor necrosis factor alpha in the aqueous humor of a rabbit corneal alkali burn model

BACKGROUND： A model of stable corneal neovascularization is a regulation mechanism for corneal neovascularation There are inhibitory and protective effects of curcumin on corneal neovascularization burned by alkali. OBJECTIVE： To explore the effects of curcumin on the expression of vascular endothelial growth factor （VEGF） and tumor necrosis factor a （TNF-a） in aqueous humor at different times after alkali burn of cornea. METHODS： Thirty-three New Zealand white rabbits were included and divided into three groups： normal group （three rabbits collected randomly）, control group （15 rabbits） and intervention group （15 rabbits）. Rabbits models of corneal neovascularization （CNV） induced by alkali burn were established in control and intervention groups. Rabbits in the control group were received normal saline on the right eyes, and rabbits in the intervention group were received curcumin on the left eyes. CNV and corneal opacity were observed by slit lamp photography. The expressions of VEGF and TNF-a in the aqueous humor before and after using curcumin were detected by Enzyme-Linked Immunosorbnent Assay. RESULTS AND CONCLUSION： No CNV was found in the control group. Compared with the control group, CNV of the intervention group was inhibited and corneal opacity was slighter （P 〈 0.05）. The expressions of VEGF and TNF-a in the aqueous humor were showed in these three groups, but the expressions of VEGF and TNF-a of the control and invention groups were significant higher than those of the normal group, and the intervention group was lower than the control group （P 〈 0.05）. These findings suggest that curcumin can inhibit the growth of CNV after alkaline burn in rabbit cornea through effectively reducing the expressions of VEGF and TNF-a in the aqueous humor after corneal alkali burn.