凋亡抑制基因Survivin在小鼠生精过程中的表达及其与生殖细胞增殖和凋亡的关系

目的：探讨凋亡抑制基因Survivin在小鼠生精过程中的表达及其与生殖细胞增殖和凋亡的关系.方法：间隔24天二次腹腔注射白消安建立小鼠生精过程的动物模型.第二次给药后随机分为1、2、3、4、6、8、10周7组,于相应时点取材.采用半定量逆转录聚合酶链反应（RT-PCR）检测Survivin mRNA的表达变化,采用免疫组织化学S-P法检测细胞核增殖抗原（PCNA）的表达,采用DNA原位末端标记TUNEL法榆测细胞凋亡.结果：Survivin基因的表达与生殖细胞增殖指数（PCNALI）呈正相关（r=0.537,P〈0.01）,与生殖细胞凋亡指数（AI）呈负相关（r＝-0.615,P〈0.01）.结论：Survivin基因的表达可促进生殖细胞的增殖,抑制生殖细胞的凋亡,有利于曲细精管生殖细胞生殖微环境稳态的维持,对正常的精子发生起重要作用.

Expression of Survivin Gene in the Recovery of Mice Spermatogenesis and Its Correlation with the Prolification and Apoptosis of Germ Cell

Objective：To investigate the expression of survivin gene in tissues of testis and its correlation with the prolification and apoptosis of germ cell in the recovery of mice spermatogenesis. Methods： Interval of 24 days intraperitoneal injection of white elimination process of pure established apart so as to establish the recovering spermotogenesis model, Testis were harvested 1 week,2 weeks,3 weeks,4 weeks,6 weeks,8 weeks, 10 weeks after the second injection. Expression of survivin gene were detected by semi quantitative RT PCR, Expression of PCNA were detected by immunohistoehemical, the apoptosis of germ cells were detected by electron microscopy and ter minal deoxynucleotidyl transterase mediated dUTP biotin nick end labeling （TUNEL） technique. Results：The expressioin of survivin gene was positively correlated with PCNA LI（r=0. 537, P〈0. 01）, it was negatively correlated with AI of germ cell （r=-0. 615 ,P〈0.01）. Conclusions：The Expression of survivin gene could increase pro lification and inhibit apoptosis of germ cell, it participates in the balance of prolification and apoptosis of germ cells, it playes an important role in the normally spermatogenesis.