Implementation of cellulomonas cholesterol oxidase for total serum cholesterol determination by the endpoint method |
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Authors: | Srisawasdi Pornpen Chaichanajarernkul Upsorn Teerakranjana Narumon Kroll Martin H |
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Affiliation: | Division of Clinical Chemistry, Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand. srisawasdiP@yahoo.com |
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Abstract: | Cellulomonas has been shown to be a good source of cholesterol oxidase in addition to Streptomyces for serum cholesterol determination by the endpoint method, inexpensive in cost, and showing excellent performance. For clinical use, we have assessed the reliability of Cellulomonas reagent for cholesterol determination. We constructed the user-defined endpoint methods on three automated analyzers. The analytical performances (linearity, precision, recovery, interference, stability, and comparison with the standardized method) of Cellulomonas cholesterol reagents were evaluated and compared to those of Streptomyces reagents. Linearity (18.1-23.3 mmol/L) and stability of reagents (6-11 weeks) depended on the analyzers being used. The average within-run and between-day % coefficients of variation (CVs) ranged from 1.44 to 2.45 and 1.98 to 2.99, respectively, and were within National Cholesterol Education Program analytical criteria (
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Keywords: | serum cholesterol Cellulomonas Streptomyces cholesterol oxidase enzymatic endpoint method implementation coronary heart disease |
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