结直肠癌K-ras基因突变与临床病理特征的相关性分析

 【摘要】 目的：研究结直肠癌组织中K-ras基因的突变情况与临床病理特性之间的相关性。目的　研究结直肠癌组织中K-ras基因的突变情况与临床病理特性之间的相关性。方法　采用直接测序法对90例结直肠癌石蜡标本进行K-ras基因突变检测，并对K-ras基因的突变情况及其与结直肠癌患者临床病理特征的关系进行统计学分析。结果　90例结直肠癌患者组织中，K-ras基因12、13密码子总突变者31例，总突变率为34.4 %。12密码子单突变21例，突变率为23.3 %；双突变1例。13密码子突变者9例，突变率为10.0 %。结直肠癌K-ras基因突变率与肿瘤部位有明显相关性（P＝0.042）。12密码子单突变和13密码子突变与临床病理参数均无明显相关性（P＞0.05）。结论　不同肿瘤部位患者的K-ras基因突变率存在明显不同。

Correlation analysis between K-ras gene mutation and clinicopathological characteristics in colorectal cancer

Objective To investigate mutations of oncogene K-ras in colorectal cancer tissues and the relationship between mutations of K-ras and clinicopathological characteristics in colorectal cancer. Methods Specimens of 90 patients with colorectal cancer were detected K-ras gene by direct sequencing. Then satistical analysis were used to analyzed the associations between K-ras mutation status and clinicopathological characteristics in colorectal cancer. Results 31 cases were detected mutation in 90 cases of eolorectal cancer specimens. The total mutation rate of K-ras was 34.4 %. There were 21 cases of single mutation occurred in codon 12. One case had double mutation in codon 12. 9 cases mutation were occurred in codon 13. There were significant difference between K-ras mutation rate and the location of tumor （P = 0.042）. The frequency of 12/13 codon mutation was no correlated with age, gender, tumor size, dukes stage, hepatic metastases, location of tumor. Conclusion The rate of K-ras mutation is correlated with the location of tumor.