CD133在肝癌组织中的表达及其临床意义

 摘要：目的 研究肝癌组织中CD133蛋白表达及其临床意义。方法 应用免疫组织化学染色方法（ABC法）检测190例原发性肝细胞肝癌患者手术切除肿瘤组织中CD133蛋白的表达,分析其与临床病理特征和预后的关系。结果 42例(22.1%)患者肿瘤组织中CD133蛋白表达阳性。肝癌组织中CD133蛋白表达与肝癌分化程度相关，42例CD133阳性肝癌中，低分化肝癌（Ⅲ+Ⅳ）为34例（17.9%），高分化肝癌（Ⅰ+Ⅱ）为8例（4.2%），差异有统计学意义（p<0.001）；同时CD133蛋白表达程度与肝癌镜下血管侵犯相关，42例CD133阳性肝癌中，存在镜下血管侵犯为33例（17.4%），无镜下血管侵犯为9例（4.7%），差异有统计学意义（p=0.016）。单因素方差分析结果表明肝癌分化程度、淋巴结转移、肉眼血管侵犯、镜下血管侵犯、肝癌TNM分期以及CD133蛋白表达情况与肝癌切除术后患者无瘤生存有关（p<0.05）。多因素方差分析结果表明CD133蛋白表达情况与镜下血管侵犯是预测肝癌切除术后患者无瘤生存的独立预测指标。生存分析表明CD133蛋白表达阳性组术后无瘤生存期明显短于CD133阴性组。结论 肝癌组织中CD133蛋白的表达与肝癌的侵袭转移及预后密切相关。

Expression of CD133 protein in hepatocellular carcinoma tissues and its clinical significance

Objective To investigate the expression of CD133 protein in primary lesions of hepatocellular carcinoma （HCC） and its clinical significance. Methods The expression of CD133 protein in 190 patients with HCC was detected by immunohistochemical staining ABC method. The correlation of CD133 protein expression with the clinicopathologic parameters and features after operation was analyzed. Results The expression positive rate of CD133 protein in cancer tissues was 22.1% （42/190）. There was significant correlation between the expression of CD133 protein and tumor differentiation （P 〈 0.001）, micro vessel invasion （P = 0.016） and hepatitis B virus infection （P = 0.024）. Univariate analysis of factors demonstrated that differentiation level, lymph node, macro vessel invasion, micro vessel invasion, TNM stage and CD133 expression were correlated with disease-free survival after surgery （all P 〈 0.05）. Multivariate analysis of factors demonstrated that both CD133 expression and micro vessel invasion were independent prognostic factors of disease-free survival after surgery. Prognostic analysis demonstrated that the disease-free survival of CD133 positive group was significantly lower than that of CD133 negative group. Conclusion The CD133 protein expression in HCC tissues is related with development, metastasis and prognosis of HCC.