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| 复合自体脂肪干细胞的脱细胞猪角膜基质移植治疗兔角膜碱烧伤 | |
| 引用本文： | 张米,尹天泉,刘源,金岩,胡丹.复合自体脂肪干细胞的脱细胞猪角膜基质移植治疗兔角膜碱烧伤[J].国际眼科杂志,2009,9(4):650-652. |
| 作者姓名： | 张米 尹天泉 刘源 金岩 胡丹 |
| 作者单位： | 1. 710032,中国陕西省西安市,第四军医大学西京医院眼科710032;中国陕西省西安市,第四军医大学口腔医学院组织工程研发中心 2. 第四军医大学西京消化病医院消化一科,中国陕西省西安市,710032 3. 第四军医大学口腔医学院组织工程研发中心,中国陕西省西安市,710032 4. 第四军医大学西京医院眼科,中国陕西省西安市,710032 |
| 基金项目： | 中国国家自然科学基金资助项目 |
| 摘 要： | 目的:研究复合脂肪干细胞的脱细胞猪角膜基质对碱烧伤角膜的治疗效果。方法:制备晾干的脱细胞猪角膜基质,取第3代体外培养的兔自体脂肪干细胞,体外种植到脱细胞猪角膜基质上,培养3d后用于板层角膜移植。结果:板层角膜移植2mo后,术眼角膜基本恢复透明,未发生排斥反应,新生血管较少,3mo后新生血管基本消退。结论:复合自体脂肪干细胞的脱细胞猪角膜基质对兔碱烧伤角膜具有良好的修复能力。 |
| 关 键 词： | 脂肪干细胞 脱细胞猪角膜基质 组织工程角膜 角膜移植 碱烧伤 |
Transplantation of acellular porcine corneal matrix complexed with autologous adipose-derived stem cells in a model of alkali burned cornea |
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| Mi Zhang,Tian-Quan Yin,Yuan Liu,Yan Jin,Dan Hu.Transplantation of acellular porcine corneal matrix complexed with autologous adipose-derived stem cells in a model of alkali burned cornea[J].International Journal of Ophthalmology,2009,9(4):650-652. | |
| Authors: | Mi Zhang Tian-Quan Yin Yuan Liu Yan Jin Dan Hu |
| Institution: | 1Department of Ophthalmology, Xijing Hospital Affiliated to the Fourth Military Medical University, Xi’an 710032, Shaanxi Pro-vince, China; 2Division 1 of Digestive Disease, Xijing Hospital Affiliated to the Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China;3Research and Development Center for Tissue Engineering, College of Stamatology of the Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China |
| Abstract: | AIM:To evaluate the effect of acellular porcine corneal matrix complexed with autologous adipose-derived stem cells for the reparation of alkali burned cornea.·METHODS: Air dried acellular porcine cornea (a-PACS) was prepared. Autologous adipose-derived stem cells were obtained and cultured in vitro. The third passage were seeded on the Bowman’s layer of a-PACS. The cell-scaffold complex was cultured for 3 days in vitro and was used for lamella transplantation.·RESULTS: The cornea of the operated eyes recovered their transparency two months later. There was no rejection and only a few new vessels were seen and was retraded in three months.·CONCLUSION: a-PACS complexed with autologous adipose-derived stem cells had the ability to repair alkali burned cornea. |
| Keywords: | adipose-derived stem cell acellular porcine corneal matrix tissue engineered cornea corneal transplantation alkali burned cornea |
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