Oxidation by thimerosal increases calcium levelsin renal tubular cells |
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Authors: | Jan Chung-Ren Jiann Bang-Ping Lu Yih-Chau Chang Hong-Tai Yu Chia-Cheng Chen Wei-Chung Huang Jong-Khing |
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Institution: | Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan 813. |
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Abstract: | The effect of thimerosal, a reactive oxidant, on cytoplasmic free Ca2+ concentrations (Ca2+]i) in Madin Darby canine kidney (MDCK) cells was explored by using the Ca2+-sensitive dye fura-2. Thimerosal acted in a concentration-dependent manner with an EC50 of 0.5 microM. The Ca2+ signal comprised a gradual rise and a sustained elevation. Removal of extracellular Ca2+ reduced 80% of the signal. In Ca2+-free medium, the Ca2+]i rise induced by 1 microM thapsigargin (an endoplasmic reticulum Ca2+ pump inhibitor) was completely inhibited by pretreatment with 5 microM thimerosal. The thimerosal (5 microM)-induced Ca2+ release was not changed by inhibition of phospholipase C with 2 microM U73122. Collectively, this study shows that thimerosal induced Ca2+]i rises in renal tubular cells via releasing store Ca2+ from the endoplasmic reticulum Ca2+ stores in a manner independent of phospholipase C activity. |
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