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Origin of the calcitonin gene-related peptide-immunoreactive nerve fibers in the rat shoulder joint
Authors:Atuo Kobayashi   Niro Yoshida   Yoshikazu Tonosaki   Shinichi Kikuchi  Yasuo Sugiura
Affiliation:(1) Department of Orthopedic Surgery, Fukushima Medical College, 960-12 Fukushima, Japan;(2) Department of Anatomy, Fukushima Medical College, 960-12 Fukushima, Japan
Abstract:The rat shoulder joint capsule is innervated by thin sympathetic and sensory nerve fibers, most of which contain calcitonin gene-related peptide (CGRP). In order to establish the origin and distribution of CGRP-immunoreactive (IR) fibers, wheat germ agglutinin-conjugated horseradish peroxidase (WGA-HRP) was injected into the shoulder joints of rats via a dorsal surgical approach. After WGA-HRP injection, the cervico-thoracic dorsal root ganglia (DRG) were removed and processed using both HRP histochemistry and CGRP immunohistochemistry. In the C4 to C7 DRG, small to medium-sized neurons (20–40 mgrm) were labeled by this combined method. The number and size of the labeled neurons were measured in the cervical 4th–7th DRG. The number of double-labeled neurons was one quarter of the total number of HRP-labeled neurons and 1/20 of the CGRP-IR neurons. Most of the double-labeled cells were located in the C6 ganglion, and the mean number of double-labeled neurons was 13 at this level. The distribution and function of the CGRP-IR fibers in the rat shoulder joint capsule are discussed.
Keywords:Wheat germ agglutinin-conjugated horseradish peroxidase (WGA-HRP)  Calcitonin gene-related peptide (CGRP)  Dorsal root ganglion Double-labeling method
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