高效液相色谱法同时测定大鼠肠灌流液中L-肌肽和酚红的浓度

目的建立HPLC方法同时测定大鼠肠灌流液中L-肌肽和酚红的质量浓度。方法采用KromasilNH2色谱柱（200mm×4．6mm，5μm），流动相为乙腈-磷酸盐缓冲液（1mmol．L^-1磷酸二氢钾，0．2mmol．L^-1磷酸氢二钾）（体积比50：50），流速1．0mL·min^-1，柱温35℃，检测波长210nm，进样量20μL。结果L-肌肽和酚红分别在质量浓度22．6～158mg·L^-1（r=0．9998）、14．2～99．4mg·L^-1（r=0．9991）与峰面积线性关系良好，2种成分回收率分别为99．1％、98．8％，RSD均〈3％。结论所用方法准确、简便、专属性好，可准确快速测定大鼠肠灌流液中L-肌肽和酚红的含量；L-肌肽的摄取过程依赖于H^＋电位梯度。

Simultaneous determination of L-carnosine and phenolsulfonphthalein in rat single pass intestinal perfusion solution by HPLC

Objective To establish an HPLC method for the simultaneous determination of L-carnosine and phenolsul- fonphthalein in rat single pass intestinal perfusion solution. Methods The separation was performed on a Kromasil NH2 column （200 mm × 4.6 mm, 5 μm） with the mobile phase of acetonitrile and phosphate buffer solution （1 mmol·L^-1 of potassium dihydrogen phosphate and 0.2 mmol·L^-1 of dipotassium hydrogen phosphate） （50 ： 50）. The flow rate was 1.0 mL·min^-1. The column temperature was 35℃. The UV detector was 210 nrlL Results The linear ranges of L-carnosine and phenolsulfonphthalein were 22.6-158 mg· L^-1 （r=0. 999 8） and 14. 2-99.4 mg·L^-1 （r=0. 999 1）, respectively. The average recovery of L-carnosine and phenolsulfonphthalein was 99.1% and 98. 8%. Conclusion The method is simple, accurate and rapid for the simultaneous determination of L-carnosine and phenolsul- fonphthalein in rat single pass intestinal perfusion solution. L-carnosine uptake process depends on the H^＋ potential gradient.