高效液相色谱法测定联磺甲氧苄啶片中3组分含量

目的建立测定联磺甲氧苄啶片含量的高效液相色谱（HPLC）法。方法采用Zorbax C18柱（150mm×4．6mm，5μm）为色谱柱，以水-乙腈-三乙胺（799：200：1，用氢氧化钠试液或冰醋酸调节pH值至5．9）为流动相，检测波长为240nm，流速为1．0mL／min，进样量为20μL，枉温为30℃。结果磺胺嘧啶和磺胺甲嗯唑质量浓度在5～100μg／mL范围内，甲氧苄啶质量浓度在2～40μg／mL范围内与峰面积线性关系良好；平均回收率磺胺嘧啶为99.91％（RSD为0.2％），磺胺甲嗯唑为99．76％（RSD为0．2％），甲氧苄啶为99．80％（RSD为0．4％）。结论HPLC法分离度好，快速、简便，可同时测定3种组分，可作为该产品的质量控制方法。

Determination of Sulfamethoxazole,Sulfadiazine and Trimethoprim Tablets by HPLC

Objective To establish the HPLC method for the determination of sulfadiazine in Sulfamethoxazole, Sulfadiazine and Trimethoprim Tablets. Methods The column packed with C18 Zorbax （150 mm ×4.6 mm, 5μm） was used. The mobile phase was water-acetonitrile- triethylamine （799 ： 200 ： 1, adjusting pH to 5.9 by sodium hydroxide reagent or glacial acetic acid） and the detection wavelength was 240 nm,the flow rate was 1.0 mL/min, the injection volume was 20 μL, the column temperature was 30℃. Results The calibration curve of sulfamethoxazole and sulfadiazine was linear in the range of 5 - 100 μg/mL,and trimethoprim was linear in the range of 2 -40 μg/mL. The average recovery rate was 99. 76% for sulfamethoxazole （ RSD =0. 2% ） ,99.91% for sulfadiazine （ RSD =0. 2% ） ,99.80% for trimethoprim （RSD =0. 4% ）. Conclusion The method is simple, accurate, and suitable for the determination of the three constituents in the compound preparation.