| HPLC法测定益骨胶囊中淫羊藿苷的含量 |
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| 引用本文： | 王冰,唐振香,郭瑞臣.HPLC法测定益骨胶囊中淫羊藿苷的含量[J].齐鲁药事,2009,28(5):271-272. |
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| 作者姓名： | 王冰 唐振香 郭瑞臣 |
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| 作者单位： | 1. 威海市立医院,山东,威海,264200
2. 山东大学齐鲁医院,山东,济南,250012 |
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| 摘 要： | 目的建立测定益骨胶囊中淫羊藿苷含量的方法.方法采用高效液相色谱法,色谱柱为Venusil XBP-C18柱(250mm×4.6mm,5μm),流动相为乙腈-水(30∶70,V/V),流速1.0mL·min-1,检测波长270nm.结果淫羊藿苷与杂质峰的分离度符合要求,淫羊藿苷在0.25～8μg·mL-1范围内峰面积与进样量线性关系良好,r=0.99349(n=5).平均加样回收率为96.07%,RSD为4.46%(n=5).结论本文建立的分析方法简便、重现性好,可用于益骨胶囊中淫羊藿苷含量测定.
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| 关 键 词： | 淫羊藿苷 益骨胶囊 含量测定 高效液相色谱法 |
Determination the content of icariin in Yigu Capsules by HPLC |
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| WANG Bing,TANG Zhen-xiang,GU Ruichen.Determination the content of icariin in Yigu Capsules by HPLC[J].qilu pharmaceutical affairs,2009,28(5):271-272. |
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| Authors: | WANG Bing TANG Zhen-xiang GU Ruichen |
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| Institution: | Weihai Municipal Hospital;Weihai 264200;1.Qilu Hospital Affiliated to Shandong University;Ji'nan 250012 |
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| Abstract: | OBJECTIVE To establish a method for the determination of icariin in Yigu Capsules. METHODS Venusil XBP-C18 column （250min×4.6mm,5μm） was used;water-acetonitrile （70：30, V/V ） were used as mobile phase, the flow rate was 1.0mL·min^-1;The detection wavelength was 270nm;RESULTS The line correlation was observed from 0.25-8μg·mL^-1 ,the averagere recoveries were 96.07%, RSD was 4.46% （n = 5）. CONCLUSION The method was simple, accurate and reproducible. |
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| Keywords: | Icariin Yigu Capsule determination HPLC |
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