ISOLATION AND CHARACTERIZATION OF ALPACA GROWTH HORMONE |
| |
| Authors: | M.J. BISCOGLIO DE JIMENEZ BONINO,O. CASCONE,A.I. ARNAO DE NU ,J.A. SANTOM ,D. S NCHEZ,R. OR ,M. VILLAVICENCIO |
| |
| Affiliation: | M.J. BISCOGLIO DE JIMENEZ BONINO,O. CASCONE,A.I. ARNAO DE NUÉ,J.A. SANTOMÉ,D. SÁNCHEZ,R. ORÉ,M. VILLAVICENCIO |
| |
| Abstract: | A simple and mild procedure is described for the isolation of pituitary growth hormone from alpaca glands. It involves extractions in buffer at pH 8. 7 and gel filtration in Sephadex G-75. An average yield of 1 mg purified product may be obtained from 1 g fresh alpaca pituitary glands. The alpaca hormone was exam ined for homogeneity by SDS-polyacrylamide gel electrophoresis, gel electro-focusing and end-group analyses. It is a protein composed of a single polypeptide chain with phenylalanine at both ends. The C-terminal sequence is -Ala-Phe. The molecular weight is approximately 21700 and the amino acid composition very similar to that observed in equine growth hormone. |
| |
| Keywords: | alpaca growth hormone pituitary purification of proteins |
|
|
