针刺结合亚低温对局灶性脑缺血再灌注大鼠Bcl-2及Bax蛋白表达的影响

目的 观察并比较针刺结合亚低温对Bcl-2、Bax表达的影响.方法 50只大鼠随机分为对照组、模型组、针刺组、亚低温组、联合组,每组10只.采用Longa线栓法改进后制作局灶性脑缺血模型,观察针刺、亚低温以及针刺结合亚低温治疗对Bcl-2、Bax表达的影响.采用免疫组化法检测指标.结果 与对照组比较,模型组Bcl-2表达显著下降,Bax表达显著升高(P＜0.01)；与模型组比较,3个治疗组Bcl-2表达显著升高,Bax表达显著下降(P＜0.01)；与其余2个治疗组比较,联合组Bcl-2表达显著升高,Bax表达显著下降(P＜0.01)；与亚低温组相比,针刺组Bcl-2表达升高不明显(P＞0.05),Bax表达下降不明显(P＞0.05).针刺与亚低温治疗效果无明显差异(P＞0.05).结论 针刺和亚低温治疗能提高Bcl-2表达,降低Bax表达,干预脑缺血,对神经元起到保护作用.在脑缺血再灌注早期,应该及时进行针刺和亚低温治疗.

Effect of Mild Hypothermia in Combination of Acupuncture on Bcl-2 and Bax Protein Expressions of Local Cerebral Ischemia/Reperfusion Rats

Objective To observe and compare the effects of acupuncture in combination of mild hypothermia therapy on the expressions of Bcl-2 and Bax. Methods Fifty rats were randomly divided into 5 groups,i.e.,the control group,the model group,the acupuncture group,the mild hypothermia group,and the combination group（acupuncture in combination of mild hypothermia therapy） ,10 in each group. Focal cerebral ischemia model was prepared with modified Longa′s suture method. The effects of acupuncture,the mild hypothermia,and the acupuncture in combination of mild hypothermia therapy on the expressions of Bcl-2 and Bax were observed. The indices were detected using immunohistochemical assay. Results Compared with the control group,the expression of Bcl-2 in the model groups significantly decreased（P0.01） ,and the expression of Bax significantly increased（P0.01） . Compared with the model group,the expression of Bcl-2 in the three treatment groups significantly increased（P0.01） and the expression of Bax significantly decreased（P0.01） . Compared with the acupuncture group and the mild hypothermia group,the expression of Bcl-2 significantly increased and the expression of Bax significantly decreased in the combination group（both P0.01） . Compared with the mild hypothermia group,increased expression of Bcl-2 and decreased expression of Bax in the acupuncture group were not obvious（P0.05） . There was insignificant difference in the therapeutic efficacy between the acupuncture group and the mild hypothermia group. Conclusions Both acupuncture and the mild hypothermia therapy could increase the expression of Bcl-2 and decrease the expression of Bax,intervene cerebral ischemia,and protect neurons. In the early cerebral ischemia/reperfusion stage,it is necessary to perform acupuncture and mild hypothermia therapy as soon as possible.