| 高效液相色谱法测定不同产地土茯苓药材中落新妇苷的含量 |
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| 引用本文： | 张笑颜,詹丽茵,曾祥腾. 高效液相色谱法测定不同产地土茯苓药材中落新妇苷的含量[J]. 中国医药导报, 2010, 7(10): 90-91 |
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| 作者姓名： | 张笑颜 詹丽茵 曾祥腾 |
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| 作者单位： | 1. 广州医学院第三附属医院,广东,广州,510150
2. 广州市医药工业研究所,广东,广州,510240 |
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| 摘 要： | 目的：建立高效液相法测定土茯苓药材中落新妇苷含量的方法。方法：采用Luna-C18（4.6mm×250mm,5μm）色谱柱;流动相：甲醇-水-冰醋酸（40∶60∶0.2）;检测波长：290nm;流速：1.0ml/min。结果：落新妇苷在0.196～1.960μg范围内与峰面积呈良好的线性关系（r=0.9999）,平均回收率为98.34%,RSD=1.80%（n=6）。结论：本方法简便、准确、重现性好,可用于土茯苓药材中落新妇苷的质量控制。
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| 关 键 词： | 高效液相法 落新妇苷 土茯苓药材 含量测定 |
Content determination of astilbin in Smilax glabra Roxb.from different provenances by HPLC |
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| ZHANG Xiaoyan,ZHAN Liyin,ZENG Xiangteng. Content determination of astilbin in Smilax glabra Roxb.from different provenances by HPLC[J]. China Medical Herald, 2010, 7(10): 90-91 |
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| Authors: | ZHANG Xiaoyan ZHAN Liyin ZENG Xiangteng |
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| Affiliation: | 1.The Third Affiliated Hospital of Guangzhou Medical University,Guangzhou 510150,China; 2. Guangzhou Pharmaceu-tical Industry Research Institute,Guangzhou 510240,China) |
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| Abstract: | Objective：To establish a method for the content determination of astilbin in Smilax glabra Roxb. by HPLC. Methods：The Luna-C18（4.6 mm×250 mm,5 μm）column was used. The mobile phases consisted of methanol,water and glacial acetic acid （40∶60∶0.2）,the flow rate was 1.0 ml/min and the detection wavelength was at 290 nm. Results：The linear ranges of astilbin was at 0.196-1.960 μg （r=0.999 9）,the average recovery was 98.34% with a RSD of 1.80% （n=6）. Conclusion：The method is fast,reliable,accurate and can be used in the quality control of astilbin in Smilax glabra Roxb.. |
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| Keywords: | HPLC Astilbin Smilax glabra Roxb. Content determination |
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