非小细胞肺癌组织MMP-2表达及MVD的研究

目的探讨非小细胞肺癌(NSCLC)组织中基质金属蛋白酶2(MMP-2)的表达与肺癌生物学行为以及血管形成之间的关系,着重分析MMP-2的表达以及新生血管形成在肺癌侵袭、转移及预后中的生物学意义。方法应用免疫组织化学方法(SP法)检测62例非小细胞肺癌组织、12例癌旁正常组织中MMP-2的表达及微血管密度(MVD)的值,并分析MMP-2的表达及MVD与组织类型、肿瘤大小、TNM分期、分化程度、淋巴结转移的相关性。结果 MMP-2在肺癌组织中的阳性表达率为62.9%,在正常组织中的阳性表达率为16.7%,两者比较差异有显著性(P0.05)。非小细胞肺癌组织中,MMP-2表达明显升高,以及MVD明显高于正常组织,与肿瘤的分化程度、临床分期、淋巴结转移有相关性(P0.05),与肿瘤的大小、病理分型无关(P0.05)。MMP-2在NSCLC中的表达以及肿瘤MVD呈显著正相关。结论检测非小细胞肺癌组织的MMP-2的表达及MVD对判断肿瘤的恶性程度和估计预后有一定的意义。

The Study on Expression of Matrix metalloproteinase-2 and Microvessel Density in non-small cell lung cancer

Objective To investigate correlations between expression of MMP2 and clinicalbiological characteristics,to analyze the relationship with invasion,metastasis and prognosis in non-small cell lung cancer.Methods High sensitive S-P immunohistochemical method was used to detect the expressions of MMP-2 and MVD in 62 cases of NSCLC and 12 cases of para-cancerous normal lung tissues.To study the relationships between their expressions and histological types of NSCLC,Tumor size,TNM stages,differentiations,lymph node metastasis and their correlations each other.Results The positive rates of MMP-2 expression was 62.9% in NSCLC,which was dramatically higher than that in a normal tissues（16.7%）（P〈0.05）.The expressions of MMP-2 and MVD was significantly correlated in NSCLC to TNM stage,differentiations and lymph node metastasis（P〈0.05）.It was no relationship with the histological types and tumor size of NSCLC（P〉0.05）.MMP-2 play important roles in non-small cell lung cancer procession,especially in tumor metastasis.MMP-2 have intimate relationship with MVD.Conclusion MMP-2 and MVD may be good preditors of metastasis and prognostic evaluation for NSCLC patients.