| 高效液相色谱法测定蒲公英颗粒中咖啡酸含量 |
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| 引用本文： | 曾秋华,刘文京,唐晓晖. 高效液相色谱法测定蒲公英颗粒中咖啡酸含量[J]. 中国药业, 2007, 16(3): 26-27 |
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| 作者姓名： | 曾秋华 刘文京 唐晓晖 |
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| 作者单位： | 北京市石景山区药品检验所,北京,100043 |
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| 摘 要： | 目的用高效液相色谱法测定蒲公英颗粒中咖啡酸的含量。方法采用C18柱，以磷酸盐缓冲液（取磷酸二氢钠1．56g，加水使溶解成1000mL，再加1％磷酸溶液调节pH值至3．8-4．0，即得）-甲醇（75：25）为流动相，检测波长为323nm。柱温为40℃，流速为1．0mL／min。结果咖啡酸质量浓度在19．54-58．62μg／mL范围内与峰面积具有良好的线性关系（r=0．9998），平均回收率为100．75％。RSD为1．0％。结论谊法操作简单，结果准确，可有效地控制蒲公英颗粒的质量。
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| 关 键 词： | 蒲公英颗粒 咖啡酸 高效液相色谱法 含量测定 |
| 文章编号： | 1006-4931（2007）03-0026-02 |
| 收稿时间： | 2006-05-23 |
| 修稿时间： | 2006-09-20 |
Determination of Caffeic Acid in Pugongying Granules by HPLC |
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| Zeng Qiuhua,Liu Wenjing,Tang Xiaohui. Determination of Caffeic Acid in Pugongying Granules by HPLC[J]. China Pharmaceuticals, 2007, 16(3): 26-27 |
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| Authors: | Zeng Qiuhua Liu Wenjing Tang Xiaohui |
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| Affiliation: | Zeng Qiuhua, Liu Wenjing, Tang Xiaohui ,(Beijing Shijingshan Institute for Drug Control, Beijing, China 100043) |
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| Abstract: | Objective To determine caffeic acid in Pugongying granules sodium dihydrogen phosphate dissolved in water to 1 000 mL, adjusting by HPLC. Methods Using C18 column and phosphonate buffer（l. 56 g with 1% phosphoric acid solution to pH was 3.8-4,0）- methanol （75：25） as mobile phase, the detection wavelength was 323 nm and the column temperature was 40℃. The flow rate was 1.0 mL/min. Results The calibration curve were linear in the range of 19. 54-58.62 μg/mL for caffeic acid （r=0.999 8）.The average recovery rate was 100. 75%, RSD was 1.0%. Conclusion This method is simple and convenient for the operation. It is very accurate and very suitable for the quality control of Pugongying granules. |
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| Keywords: | Pugongying granules caffeic acid HPLC content determination |
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