氯膦酸二钠脂质体对胰腺炎肾损伤的保护作用

目的 探讨氯膦酸二钠脂质体(liposomal clodronate)对大鼠重症急性胰腺炎.肾损伤的干预效应.方法SD大鼠48只,随机分为三组:对照组(C)、SAP+空白脂质体组(P)、SAP+Clodronate-脂质体治疗组(T).经大鼠胰腺被膜下均匀注射5%牛磺胆酸钠制备SAP模型,C组仅胰腺被膜下注射等量生理盐水,P组经尾静脉注射空白脂质体,T组经尾静脉注射Clodronate-脂质体.在制模后2,6 h取血,测定血清AMS,BUN,Cr,IL-6及IL-12,观察肾脏和胰腺组织的病理学变化.结果 与C组比较,P组2,6 h胰腺及.肾病理改变明显加重.P组各时相BUN,Cr较C组明显增高(P<0.01),IL-6,IL-12在2,6 h明显增加(P<0.01).与P组比较,T组在制模后2,6 h胰腺及肾的病理损害较P组有所减轻,T组各时相血浆Cr较P组明显降低(P<0.01),BUN 6 h降低有显著意义(P<0.05),IL-6,IL-12在2,6 h明显降低(P<0.01).结论炎症介质的过度释放在SAP肾组织损伤中起着重要的作用,Clodronate经脂质体包裹对胰腺及肾损害具有保护作用.

Study on protective effects of lipsomal clodronate on renal injury acute pancreatitis

Objective To investigate the protective effects of lipsomal clodronate on renal injury in rats with severe acute pancreatifis and the assessment of renal injury. Method Totally 48 rats were randomly divided into three group:normal control group (C);SAP group, in which rats were treated with pure liposomal (P);treatment group, in which SAP rats were treated with liposomal clodronate disodium(T). The SAP model of rat was induced by injection of 5 % sodium taurochohte beneath the pancreatic membrane. Rats of normal control group received isovolumetric injections of 0.9% physiological saline solution instead of sodium taurocholate. Blood samples were collected to measure AMS,BUN,Cr,IL-6 and IL-12 at 2 hors, 6 hours after SAP. At the same time, the samples of pancreatic and renal tissues were taken for observing the pathological changes. Results Compared with controlgroup, serious renal and pancreatic damages were found in group P, and the AMS, BUN, Cr levels elevated signifi-candy (P < 0.01). Compared with group P,the renal and pancreatic damages were attenuated in group T, and the levels of Cr and AMS decreased significantly (P < 0.01), and the IL-6, IL-12 were decreased at 2 hours and 6 hours (P < 0.01). The BUN decreased significantly at6 hours (P < 0.05). Conciusions Excessive release of inflammatory mediator play an important role in renal injury in SAP. Lipsomal clodronate disodium can alleviate the damage of pancreas and kidney.