睾酮对血管内皮生长因子及其受体在大鼠前列腺组织中表达的影响

目的　探讨睾酮对血管内皮生长因子 (VEGF)及其受体之一 (FL K- 1)在前列腺组织中表达的影响。方法　分别在摘取大鼠睾丸后 7天及以后注射不同剂量的睾酮 ,HE染色观察前列腺的组织学变化 ,免疫组化SP法检测 VEGF和 FL K- 1在大鼠前列腺腹侧叶的蛋白表达。结果　正常大鼠前列腺可见多种生长状态上皮 ,VEGF和 FL K- 1蛋白在增生活跃上皮及间质的血管内皮上有阳性表达 ;去势可导致大鼠前列腺腹侧叶重量减轻(P<0 .0 5 ) ,组织学表现为上皮组织萎缩 ,VEGF和 FL K- 1在前列腺上皮中的蛋白表达明显降低 (P<0 .0 5 ) ;注射睾酮后 ,前列腺重量逐渐增加 (P<0 .0 5 ) ,组织学表现为上皮增生逐渐活跃 ,VEGF和 FL K- 1在前列腺上皮中的蛋白表达逐渐增强 (P<0 .0 5 )。结论　睾酮是维持大鼠前列腺腹侧叶生长和发育所必需的 ,睾酮可能通过正向调节VEGF及其受体 (FL K- 1)的蛋白表达来刺激前列腺组织的生长

Effects of Testosterone on VEGF and FLK-1 Protein Expression in the Ventral Prostate of Rat

Objective To investigate the effects of testosterone on VEGF and FLK-1 protein expression in the ventral prostate of Rat. Method SD rats were castrated, and 7 days later they were injected with different doses of testosterone. The histological changes were observed with the use of HE staining, and the protein expression of VEGF, FLK-1 were measured by immunohistochemical test. Results In the normal ventral prostate, different growth forms of epithelium were observed. In the castrated group, the proteins of VEGF, FLK-1 were positive in growth active epithelium and vascular endothelium; the weight of prostate decreased(P<0.05); the histological appearance was epithelial atrophy, and there was significantly decreased protein expression of VEGF,FLK-1 in the prostatic epithelium (P<0.05). It was found that testosterone injection stimulated prostatic hyperplasia gradually The histological changes were epithelial progressive proliferation. And a gradually increased protein expression of VEGF,FLK-1 in the prostatic epithelium was detected (P<0.05). Conclusion Testosterone can stimulate prostatic growth probably by upregulating the protein expression of VEGF and FLK-1.